Leica DMi8
Leica DMi8
Modalities: invert, widefield, live cell
Overview
A fully automated widefield system for fluorescence and brightfield imaging.
The DMi8 is a fully automated widefield system for fluorescence and brightfield imaging. It is equipped with a full range of objectives, monochrome and colour cameras and filtersets to enable fluorescence imaging of up to four distinct channels from UV to far red excitation.
Stage and z are also motorised, allowing multichannel z stacks in multiple positions or tilescans, plus in combination with an Okolab on-stage incubator, live cell imaging is also possible.
Imaging Modes:
- 2D (xy) or 3D (xyz), multichannel with timelapse and/or tiling and/or multiple locations
Specification
Here you can find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslips |
Other Modes |
Immersion |
5 | 0.15 | Air | ||
10 | 0.4 | 0.17 | Air | |
20 | 0.8 | 0.17 | Air | |
40 | 0.85 | 0.17 | Air | |
40 | 1.3 | 0.17 | Oil | |
63 | 1.4 | 0.17 | Oil | |
100 | 1.44 | 0.17 | DIC | Oil |
Filters (epi)
Cube |
Example Fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
Leica DAPI | DAPI, Hoechst | 325-375 | 400 | 435-485 | |
Leica GFP | GFP, FITC,AF488 | 450-490 | 500-550 | ||
Leica Rhod | Cy3, AF555 | 541-551 | 565-605 | ||
Leica TXR* | AF568, AF594 | 540-580 | 585 | 592-668 | |
Leica CY5 | CY5, AF647 | 560-650 | 662-738 | ||
Leica mK-Red | mK-red | 550-570 | 602-682 | ||
Leica mK-Green | mK-green | 420-460 | 602-682 |
*fitted on request
Light Sources
- Lumencor SOLA SE365 LED light engine for epifluorescence
- White LED for transmission
Cameras
- Hamamatsu Flash 4 v2, (monochrome 4MP sCMOS)
- Leica DFC450 (colour 5MP CCD)
Location
Biogerontology Building, Room F20
Health Innovation Neighbourhood
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.