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Nikon NiE

Modalities: upright, widefield

Overview

The Nikon NiE is a semi-automated microscope.

The Nikon NiE microscope can be used to view slides. The camera is an Andor Zyla 5.2.

The filters, condenser, motorized stage and z drive are all automated. This allows for multichannel, z stack imaging to be performed.

The system is run through Nikon’s versatile Elements software package. It also incorporates wizards to allow the automated capture and analysis of fluorescence images.

Imaging modes

  • Widefield fluorescence
  • Phase Contrast
  • XYZ (3D)

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification

NA

Coverslip

Other Modes

Immersion

4 0.2     Air
10 0.45 0.17   Air
20 0.7 0.17   Air
40 0.95 0.17   Air
40 1.3 0.17   Oil
100 1.4 0.17   Oil

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part Number

Nikon DAPI DAPI, Hoechst 340-380 400 435-485  
Nikon FITC GFP, FITC, AF488 465-495 505 515-555  
Nikon TRITC TRITC 527-552 565 557-633  
Nikon Cy3 Cy3 515-555 565 570-610  
Nikon Cy5 Cy5, AF647 590-650 660 665-735  

Light source (epi)

CoolLed pE300 (Broad spectrum LED fluorescent light)

Detectors (camera)

Andor Zyla 5.5-Monochrome (for fluorescent imaging)

Location

NMJ Facility, Cookson Building, Dental School – G32

Medical School
Framlington Place
Newcastle University 
Newcastle upon Tyne 
NE2 4HH

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 
Make a booking

Training video

This tutorial video shows you how to operate the microscope including start-up, software introduction, image capture, cleaning procedure and shutdown.