Nikon TiE
Modalities: invert, widefield, high content
Overview
The microscope (Nikon TiE) is a fully automated and employs hardware and software focal drift correction.
The system is equipped with both a mono (Andor Zyla 5.5 Mono) as well as with a color camera (Nikon DS-Fi1) which has been configured for multi-well plate-based screening applications but can be used to view slides with the appropriate stage insert. This system is equipped with an enviromental chamber for Live cell imaging with temperature and CO2 control.
The system is run through Nikon’s versatile Elements-JOBS software package and incorporates wizards to allow the automated capture and analysis of both fluorescence and colour images.
Imaging modes
- Multi point x,y (2D) with tiling
- Volume x,y,z (3D)
- Time lapse x,y,z,t (4D)
- Fluorescence
- DIC
- Brightfield imaging
- Live Cell imaging
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
|---|---|---|---|---|
| 4 | 0.2 | Air | ||
| 10 | 0.45 | 0.17 | DIC | Air |
| 20 | 0.75 | 0.17 | Air | |
| 40 | 0.6 | 0.17 | DIC-Ph2 | Air |
| 40 | 0.95 | 0.17 | Air |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
|---|---|---|---|---|---|
| Nikon DAPI | DAPI, Hoechst | 340-380 | 400 | 435-485 | |
| Chroma 49002-ET | GFP, FITC, AF488 | 450-490 | 495 | 500-550 | |
| Chroma 49003-ET | EYFP | 490-510 | 515 | 520-550 | |
| Nikon G2A | mCherry, AF594 | 510-560 | 565 | 590 (LP) | |
| Chroma 49006-ET | Cy5, AF647 | 590-650 | 650 | 665-735 |
Light source (epi)
CoolLed pE 340fura (340nm, 380nm)
Sola SE (Broad spectrum LED light)
Detectors (PMTs and cameras)
Andor Zyla 4.2 sCMOS
Nikon DS-Fi1