VisiTech iSIM
Modalities: invert, confocal, live cell
Overview
The VisiTech iSIM confocal is an array-scanning confocal microscope based around a fully motorized, inverted Nikon TiE body.
This microscope provide live cell incubation with a high speed super resolution imaging system capable of capturing super resolution images in real time up to 1000 frames per second.
The system has two Hamamatsu Orca Flash 4 sCMOS monochrome cameras for simultaneous two colour fluorescence imaging.
The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks.
Imaging modes
- Multi point x,y (2D) with tiling,
- Volume x,y,z (3D),
- Time lapse x,y,z,t (4D)
- Live cell
- Superresolution
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
|---|---|---|---|---|
| 10 | 0.3 | 0.17 | DIC L/N1 | Air |
| 20 | 0.75 | 0.17 | DIC N2 | Air |
| 60 | 1.25 | 0.17 | DIC N2 | Water |
| 100 | 1.49 | 0.17 | DIC N2 | Oil |
Filters(epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
|---|---|---|---|---|---|
| Nikon DAPI | DAPI, Hoechst | 340-380 | 400 | 435-485 | |
| Nikon FIRC | GFP, FITC, AF488 | 465-496 | 505 | 515-555 | |
| Nikon TxRED | Texas Red, Af594 | 540-580 | 595 | 600-660 |
Light source (epi)
Intensilight (Broad spectrum fluorescent light)
Laser lines
405nm, 488nm, 561nm, 640nm
Detectors (cameras or PMTs)
DUAL Hamamatsu ORCA-Flash-4.0 V2