Zeiss AxioExaminer (NMJ)
Zeiss AxioExaminer
Modalities: upright, widefield, electrophysiology
Overview
The Zeiss AxioExaminer is an upright microscope compatible with electrophysiology techniques.
This equipment was funded by [Wellcome Trust, 2017], please reference the funder and [208339/Z/17/Z], in your publication.
This system offers a high quality imaging system for fluorescent imaging combined on an electrophysiology platform for combined experiments. It has superabundant space for specimen and experimental tools.
Imaging modes
- Brightfield transmitted light (phase and DIC)
- Wide field fluorescence
- Imaging + electrophysiology combining mode
Specification
Here you can find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
Currently in use: |
||||
5 | 0.16 | Air | ||
10 | 0.45 | 0.17 | DIC | Water Dipping |
40 | 1 | 0.17 | DIC | Water Dipping |
40 | 0.75 | 0.17 | Water Dipping | |
Additional available lenses: |
||||
2.5 | 0.12 | 0.17 | Air | |
10 | 0.3 | Water Dipping | ||
10 | 0.5 | Water Dipping | ||
63 | 1.4 | Oil | ||
63 | 0.9 | 0.17 | DIC | Water Dipping |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
Zeiss 90 HE LED | DAPI | 370-400 | 405 | 410-440 | |
FITC | 450-488 | 493 | 499-529 | ||
AF5686 | 540-570 | 575 | 580-604 | ||
AF647 | 615-643 | 653 | 659-759 | ||
Zeiss 38 HE |
GFP, FITC, AF488 | 450-490 | 495 | 500-550 | |
Zeiss 63 HE | AF568, mCherry, TRITC | 560-584 | 590 | 598-660 | |
Light source (epi)
Colibri’ 7- LED fluorescent light (385/30, 423/44, 469/38, 511/44, 555/30, 590/27, 631/333)
Detectors (cameras)
Hamamatsu ORCA-Flash-4.0 V3 (Monochrome for fluorescence imaging)
Location
NMJ Facility, Cookson Building, G33
Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH
Enter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.