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Zeiss AxioImager with Apotome (System 1)

Modalities: upright, widefield

Overview

The Zeiss AxioImager is a high quality wide field imaging system that offers three modes of operation:

  • brightfield transmitted light (phase and DIC)
  • wide field fluorescence
  • apotome structured illumination (confocal like) fluorescence

The system utilises two separate camera systems for colour and monochrome imaging.

The Apotome (structured illumination) mode allows users to collect confocal-like images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.

The illumination source is Colibri 7.

This system is capable of visualising Cy7 as it has the appropriate filter and wide spectrum lightsource. 

The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.

Analysis of data and images can be carried out using the Zen Blue software which includes quantification and colocalisation modules.

Imaging modes

  • Wide field fluorescence
  • Brightfield
  • Multi-point x,y (2D) with tiling
  • Volume x,y,z (3D)
  • Structured illumination (Apotome, fluorescence only)
  • Phase contrast
  • DIC 

Specification

Here you can find the specifications for this microscope.

 

Objectives

Magnification

NA

Coverslip

Other modes

Immersion

2.5 0.075     Air
10 0.3 0.17   Air
20 0.8 0.17   Air
40 0.75 0.17 DIC Air
63 1.4 0.17 DIC Oil
100 1.4 0.17 Ph3 Oil

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part Number

DIC/Analyser          
Zeiss 112 DAPI, AF488, AF555, AF647, Cy7 335-385 405+439+ 575 + 654 + 761 PBP 425/30 + 514/31 + 592/25 + 681/45 + 785/38  
Zeiss 38 HE GFP, FITC, AF488 450-490 495 605/70  
Zeiss 43 Cy3, AF555 545/25 570 630/75  
Zeiss 20 HE TRITC, Rhodamine 540-552 560 575-640  
Zeiss 49 DAPI 365 395 445/50  
Zeiss 50 Cy5, AF660 640/30 660 690/50  
Zeiss 91 CFP, YFP, mCherry 423/44 450+538+610 TBP 467/24+555/25+687/145  

*Discontinued for the newest SpOr-B-000 with a slightly different dichroich (FF506-Di03 instead of FF506-Di02)

Light source (epi)

Colibri 7 (385nm/430nm/475nm/555nm/630nm/735nm)

Detectors (cameras)

Flash4 camera

Zeiss MRc (Colour for brightfield HC)

Location

William Leech Building, room M2.149

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 

Training

Here you find useful training videos for the system.

Zen Blue software tutorial

Explore Zen