Zeiss AxioImager with Apotome (System 2)
Modalities: upright, widefield
Overview
The Zeiss AxioImager is a high quality wide field imaging system that offers three modes of operation:
- brightfield transmitted light (phase and DIC)
- wide field fluorescence
- apotome structured illumination (confocal like) fluorescence
The system utilises two separate camera systems for colour and monochrome imaging.
The Apotome (structured illumination) mode allows users to collect confocal-like images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.
A range of fluorescent filters are available which cover the commonly used dyes:
- DAPI
- FITC
- TRITC
- CY5
The illumination source is a Colibri LED.
The microscope has as a motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.
Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules.
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
|---|---|---|---|---|
| 5 | 0.16 | Air | ||
| 10 | 0.45 | 0.17 | Ph1 | Air |
| 20 | 0.8 | 0.17 | Ph2 | Air |
| 40 | 0.95 | 0.17 | Ph3 | Air |
| 63 | 1.4 | 0.17 | DIC | Oil |
| 100 | 1.4 | 0.17 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
|---|---|---|---|---|---|
| Zeiss 49 | DAPI, Hoechst | 335-385 | 395 | 420-470 | |
| Zeiss 38 HE | GFP, FITC, AF488 | 450-490 | 495 | 500-550 | |
| Zeiss 23 | FITC | 475-495 | 500 | 515-530 | |
| TRITC | 540-552 | 560 | 580-630 | ||
| Zeiss 63 HE | AF568, mCherry, TRITC | 560-584 | 590 | 598-660 | |
| Zeiss 50 | CY5, AF647 | 625-655 | 660 | 665-715 |
Light source (epi)
Colibri 1- LED fluorescent light (365,470,555,625)
Detectors (cameras)
Flash 4 camera
Zeiss 105 (Colour for brightfield HC)