Zeiss AxioVert 2 East Wing
Modalities: invert, widefield
Overview
The Zeiss AxioVert 2 offers two modes of observation, Brightfield transmitted light (phase & DIC) and Widefield fluorescence.
The Zeiss AxioVert 2 system utilises a monochrome imaging camera.
A range of fluorescent filters are available which cover the commonly used dyes:
- DAPI
- FITC
- TRITC and CY5
The illumination source is a HBO100 mercury arc lamp.
The microscope has a heated stage insert for live samples.
Imaging modes
- Wide field fluorescence
- Brightfield
- Phase contrast
Specification
Here you can find the specifications for this microscope.
Objective
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
|---|---|---|---|---|
| 5 | 0.15 | 0.17 | Ph1 | Air |
| 10 | 0.32 | 0.17 | Ph1 | Air |
| 20 | 0.4 | 0.17 | Ph2 | Air |
| 40 | 0.6 | 0.17 | Korr Ph2 ELWD | Air |
| 63 | 0.75 | 0.17 | Korr Ph2 ELWD | Air |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
|---|---|---|---|---|---|
| Zeiss 02 | DAPI, Hoechst | 335-390 | 395 | 420 (LP) | |
| Zeiss 47 HE |
CFP |
424-448 | 455 | 460-500 | |
| Zeiss 13 |
eGFP |
460-480 | 495 | 505-530 | |
| Zeiss 46 HE | FITC, AF488 | 487-512 | 515 | 520-550 | |
| Zeiss 15 | dTomato, MT Orange | 540-552 | 580 | 590-LP |
Light source (epi)
HBO 100 (Broad spectrum fluorescent light)
Detectors (cameras)
Zeiss AxioCam HRc (Colour camera used both for fluorescence and brightfield HC)