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Zeiss CellDiscoverer7

Modalities: widefield, live cell, high content, long time-lapse

Overview

Our flagship high content system running Zeiss’ Zen software suite is a fully automated widefield microscope system complete with 40 place room temperature and 40 place incubated plate storage system with integrated robotic loader and scheduling system. Using a combination of air and water immersion lenses and a multi wavelength LED illumination system the CD7 allows for automated multiplate and multi slide imaging captured on a highly sensitive Hamamatsu Fusion sCMOS camera. The system also allows for a conditional or guided acquisition experiment to be designed and automatically analysed, further increasing the automation possibilities for large scale plate or slide arrays.

Specification

Objectives

Magnification

NA

Coverslip

Other modes

Immersion

5 0.35 0.17   Air
20 0.7 0.17   Air
 50 1.2 0.17   Air

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part number

Zeiss 49 DAPI, Hoechst 335-385  395 420-470   

Zeiss 38 HE

GFP, FITC, AF488 450-490 495 500-550  

Zeiss 91 HE LED

AF594/AF532/CFP/mCherry   450, 538, 610 457-477, 545-566, 617-765  

Zeiss 90 HE LED

DAPI/FITC/AF458/AF647   450, 493, 575, 653 410-440, 499-529, 580-604, 659-759  

Zeiss 568

AF568/mCherry/TRITC     574-631  

Zeiss 647

AF647     765-885  

Light Source (Epi)

Colibri 7 LED (358/30; 423/44; 469/38; 511/44; 567/30; 590/27; 625/33

Camera 

Monochrome CCD Zeiss 506

Monochrome sCMOS Hamamatsu Fusion

Location

Leech Building, M2.154

Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 

Training video

This tutorial video shows you how to operate the microscope including start-up, image capture, cleaning procedure and shutdown.