Zeiss LSM880
Zeiss LSM880
Modalities: invert, confocal, live cell, airyscan
Overview
The Zeiss LSM 880 with Airyscan Fast is a confocal laser scanning microscope able to generate three-dimensional images of large specimen at super-resolution.
The system consists of an inverted microscope suitable for live cell imaging as well as fixed samples.
The Airyscan system incorporates a novel spatial detector, the ‘Airyscan detector’, that increases spatial resolution 1.7x in each dimension (140 nm lateral and 400 nm axial resolution) or have the capabilities to achieves 4x faster temporal super-resolution imaging and requires 8x lower laser power while increasing the sensitivity, especially suited for dynamic life cell imaging. Due to the full environmental camber to control temperature and CO2 this microscope is perfect for life cell imaging as well as temperature sensitive studies.
Imaging modes
- Multipoint x,y with tiling (2D)
- Volume x,y,z (3D)
- Fluorescence
- DIC
- Time-lapse x,y,z,t (4D)
- Z-stack and multi-color imaging of cell cultures, living sections, organs.
- Imaging + electrophysiology combining mode
- SuperResolution (AiryScan)
Specification
Here you find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
10 | 0.45 | 0.17 | Air | |
20 | 0.8 | 0.17 | Air | |
40 | 1.2 | 0.17 | W Korr FCS | Water |
40 | 1.3 | 0.17 | DIC UV-IR | Oil |
63 | 1.4 | 0.17 | DIC | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
Zeiss 49 | DAPI, Hoechst | 335-385 | 395 | 420-470 | |
Zeiss 38 HE |
GFP, FITC, AF488 | 450-490 | 495 | 500-550 | |
Zeiss 43 HE | CY5, AF647 | 625-645 | 660 | 675-715 | |
Light source (epi)
HXP120 - Broad spectrum fluorescent light
Laser lines
355nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm
Detectors
GaAsP and AiryScan
Location
West Wing, International Centre for Life, room B160
Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ
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Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.