Zeiss LSM880 NLO Multiphoton
Modalities: invert, confocal, multiphoton, airyscan
Overview
Zeiss LSM 880 upright confocal multiphoton microscope with Airyscan detector.
The LSM880 is a point scanning confocal microscope built on an inverted microscope body. There are a range of objectives, optimally chosen for in vivo or high resolution multiphoton microscopy. The microscope is all automated except for the condenser. The focus drive can be driven by the software, and a fast piezo drive is available (for z stacks up to 400 nm). Optionally, the system can be inverted to allow using the microscope as an upright imaging system using an LSMTech Inverterscope.
For confocal imaging, there are 6 continuous wave (CW) excitation laser lines and 2 pulsed lasers, one fixed at 1040 nm plus one variable (680-1300 nm) for Non Linear Optical (NLO)/ multiphoton microscopy.
For detection, a choice of 4 internal detectors: 2 photomultiplier tubes (PMTs) and 1 GaAsP detector in the scanhead using a shared pinhole, plus an Airyscan 32x GaAsP array. The Airyscan module can be used in several modes, enabling super-resolution, virtual pinhole or Airyscan fast modality. For multiphoton imaging, there is also a linear array of 4 external non descanned detectors (NDDs), 2 PMTs and 2 GaAsPs with a choice of filters in reflective position.
The system is equipped with a blacked out heated incubator and CO2 module to enable stable operating temperature for in vivo imaging.
Specification
Here you find the specification for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
|---|---|---|---|---|
| 10 | 0.45 | 0.17 | Water | |
| 25 | 0.8 | 0.17 | Multi immersion, water/ oil/ silicone or glycerine with or without coverslip | |
| 32 | 0.8 | 0.17 | Water | |
| 100 | 1.45 | Oil | ||
| 40 | 1.3 | 0.17 | Oil | |
| 63 | 1.4 | Oil | ||
| 40 | 1.3 | Oil | ||
| 20 | 0.7 | Air | ||
| 10 | 0.45 | Ai |
Filters (epi)
For the three single internal detectors (2x PMT, 1x GaAsP), freely moveable bandpass or longpass filters from 380-760nm.
For the internal Airyscan detector the following dual filtersets are available:
- BP 420-480 + BP 495-550
- BP 420-480 + BP 495-620
- BP 465-505 + LP 525
- BP 495-535 + LP 555
- BP 495-550 + LP 570
- BP 555-620 + LP 645
For the NDDs, the following filtercubes are available:
|
EM. filter 1 |
Dichroic |
EM. filter 2 |
|---|---|---|
| BP 380-430 | LP 445 | empty |
| SP 485 | LP 490 | empty |
| BP 500-530 | LP 550 | BP 570-610 |
| BP 500-550 | LP 555 | empty |
| BP 570-610 | LP 625 | empty |
| BP 645-710 | Mirror | empty |
| BP 465-515 | Mirror | empty |
| EF 485 | LP 490 | empty |
| BP 380-430 | LP 445 | empty |
| BP 453-467 | LP 470 | empty |
| BP 517.5-525.5 | LP 532 | BP 580-620 |
Other filtersets for the NDDs can be built upon request if none match your needs.
Lasers lines
CW lasers
- Argon, providing 458, 488 and 514nm
- HeNe 543 nm
- HeNe 633 nm
- 405 nm
Pulsed Lasers
- 1040 nm, 80 MHz, 100 fs.
- 680-1300 nm, 80 MHz, ~100fs
Detectors
- Internal 2 x PMT, 1 x GaAsP and 1 x Airyscan 32-GaAsP array
- External, 2 x PMT and 2 x GaAsP NDDs