Zeiss AxioImager with Apotome (System 5)
Modalities: upright, widefiled
Overview
The Zeiss AxioImager (system 5) is a high quality widefield imaging system that offers three modes of operation:
- Brightfield transmitted light
- Widefield fluorescence
- Apotome structured illumination (confocal-like) fluorescence
The system utilises two separate camera systems for colour and monochrome imaging.
The Apotome (structured illumination) mode allows users to collect confocal-like images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.
A range of fluorescent filters are available which cover the commonly used dyes:
- DAPI/GFP/Cy3/Cy5
- mPlum
- Cy7
the illumination source is a wide spectrum alignment free LED (colibri) system.
The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.
Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules.
Imaging modes
- Wide field fluorescence
- Brightfield
- Multi-point x,y (2D) with tiling
- Volume x,y,z (3D)
- Structured illumination (Apotome, fluorescence only)
- Phase contrast
- DIC
Specification
Here you can find the specifications for this microscope.
Objectives
| Magnification | NA | Coverslip | Other modes | Immersion |
|---|---|---|---|---|
| 2.5 | 0.075 | Air | ||
| 5 | 0.16 | 0.17 | Ph1 | Air |
| 10 | 0.45 | 0.17 | Ph1 | Air |
| 20 | 0.8 | 0.17 | Ph1 | Air |
| 40 | 1.3 | 0.17 | Ph2 | Oil |
| 63 | 1.4 | 0.17 | Ph3 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
|---|---|---|---|---|---|
| Zeiss 90 | DAPI, GFP,Cy3,Cy5 | BP 385/30 BP 469/38 BP 555/30 BP 631/33 |
QBS 405 + 493 + 575 + 653 | QBP 425/30+514/30+592/25+709/100 | 489090-9110-000 |
| Zeiss 64 | mplum | BP 587/25 (HE) | FT 605 (HE) | BP 647/70 (HE) | 489064-0000-000 |
| Shemrock Cy7 |
Light source (epi)
Colibri 7- LED fluorescent light (365,470,555,625)
Detectors (cameras)
Zeiss Axiocam 503 (Monochrome for fluorescence imaging)
Zeiss Axiocam 105 (Colour for brightfield HC)