Zeiss AxioImager (System 5)
Zeiss AxioImager with Apotome (System 5)
Modalities: upright, widefiled
Overview
The Zeiss AxioImager (system 5) is a high quality widefield imaging system.
The Zeiss AxioImager is a high quality imaging system that offers three modes of operation:
- Brightfield transmitted light
- Widefield fluorescence
- Apotome structured illumination (confocal-like) fluorescence
The system utilises two separate camera systems for colour and monochrome imaging.
The Apotome (structured illumination) mode allows users to collect confocal-like images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.
A range of fluorescent filters are available which cover the commonly used dyes:
- DAPI/GFP/Cy3/Cy5
- mPlum
- Cy7
the illumination source is a wide spectrum alignment free LED (colibri) system.
The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.
Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules.
Imaging modes
- Wide field fluorescence
- Brightfield
- Multi-point x,y (2D) with tiling
- Volume x,y,z (3D)
- Structured illumination (Apotome, fluorescence only)
- Phase contrast
- DIC
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification | NA | Coverslip | Other modes | Immersion |
2.5 | 0.075 | Air | ||
5 | 0.16 | 0.17 | Ph1 | Air |
10 | 0.45 | 0.17 | Ph1 | Air |
20 | 0.8 | 0.17 | Ph1 | Air |
40 | 1.3 | 0.17 | Ph2 | Oil |
63 | 1.4 | 0.17 | Ph3 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
Zeiss 90 | DAPI, GFP,Cy3,Cy5 | BP 385/30 BP 469/38 BP 555/30 BP 631/33 |
QBS 405 + 493 + 575 + 653 | QBP 425/30+514/30+592/25+709/100 | 489090-9110-000 |
Zeiss 64 | mplum | BP 587/25 (HE) | FT 605 (HE) | BP 647/70 (HE) | 489064-0000-000 |
Shemrock Cy7 |
Light source (epi)
Colibri 7- LED fluorescent light (365,470,555,625)
Detectors (cameras)
Zeiss Axiocam 503 (Monochrome for fluorescence imaging)
Zeiss Axiocam 105 (Colour for brightfield HC)
Location
Biomedicine West Wing, International Centre for Life, room B162
Newcastle UniversityInternational Centre for LifeCentral ParkwayNewcastle upon TyneNE1 3BZEnter your postcode or address to find directions
Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.
Training
Here you find useful training videos for the system.