Zeiss AxioObserver Z1
Modalities: inverted, widefiled
Overview
The Zeiss AxioObserver Z1 is a high quality widefiled imaging system specific for live imaging that offers two modes of operation:
- Brightfield transmitted light
- Widefield fluorescence
The system utilises two separate camera systems for colour and monochrome imaging.
A range of fluorescent filters are available which cover the commonly used dyes:
- DAPI/GFP/mCherry
- Red Fluorescent protein
- AF546
- GFP
- DAPI
the illumination source is a wide spectrum alignment free LED (colibri) system.
The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.
Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules.
Imaging modes
- Wide field fluorescence
- Brightfield
- Multi-point x,y (2D) with tiling
- Phase contrast
- DIC
Specification
Here you can find the specifications for this microscope.
Objectives
| Magnification | NA | Coverslip | Other modes | Immersion |
|---|---|---|---|---|
| 5 | 0.15 | 0.17 | Ph1 | Air |
| 10 | 0.3 | 0.17 | Ph1 | Air |
| 20 | 0.4 | 0.17 | Korr | Air |
| 40 | 0.6 | 0.17 | Korr | Air |
| 40 | 1.3 | 0.17 | Ph3 | Oil |
| 63 | 1.4 | 0.17 | Ph3 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part number |
|---|---|---|---|---|---|
| Zeiss 92 | DAPI,GFP,mCherry | BP 385/30 BP 469/38 BP 591/27 |
TBS 405+493+610 | BP 425/30 BP 525/50 (HE) BP 649/65 |
489092-9010-000 |
| Zeis | RFP | BP 572/25 (HE) | FT 590 (HE) | BP 629/62 (HE) | 489063-0000-000 |
| Zeiss 15 | AF546 | BP 546/12 | FT 580 | LP 590 | 488015-0000-000 |
| Zeiss 13 | GFP | BP 470/20 | FT 495 | BP 505-530 | 488013-0000-000 |
| Zeiss 49 | DAPI | G 365 | FT 395 | BP 445/50 | 488049-9901-000 |
Light source (epi)
Colibri 2- LED fluorescent light (365,470,555,625)
Detectors (cameras)
Zeiss Axiocam 503 (Monochrome for fluorescence imaging)
Zeiss Axiocam 105 (Colour for brightfield HC)