Staff Profile

Introduction

I am a complement biologist and B cell immunologist with a focus on kidney and eye disease.

My research group is part of the Complement Therapeutics Research Group which includes the groups of Prof's Claire Harris (honorary), Neil Sheerin and David Kavanagh.

please see our renal research in Newcastle

I am head of autoimmune research and analysis at the National Renal Complement Therapeutics Centre (NRCTC), RVI, Newcastle-upon-Tyne.

Click here to see the NRCTC web pages

My current interests are the role of anti-complement protein autoantibodies in aHUS and C3G (MPGN). The development of Factor H based therapeutics, primarily for the treatment of C3G and aHUS. This sits along side a long standing interest in the role of CR2 in B cell function and the use of complement proteins as adjuvants in vaccine design, as well as research around solving the problems of the cold chain.

Background

1989-1993 Obtained an upper class 2 degree (2\1) in B.Sc. (Hons) Immunology from the University of Glasgow

1993- 97 Obtained Ph.D., based on “Cell resistance and recovery after complement attack: The role of CD59” under the supervision of Professor B. Paul Morgan & Dr Carmen W. van den Berg.

1997-2000 Awarded a prestigious Wellcome Trust prize international fellowship to generate human complement receptor 2 transgenic mice within Prof. V. Michael Holers laboratories in the Dept. of Medicine and Immunology, UCHSC, Denver, CO, USA.

2000-0ct 2002 In continuation of the Wellcome Trust Prize International Fellowship, I returned to Prof. B. Paul Morgan’s laboratories in Cardiff, Wales to fully develop my research on the hCR2 transgenic mice.

Oct 2002- Oct 2006 Obtained a Wellcome Trust research career development fellowship (RCDF), at the UWCM (now Cardiff University) where I established a small research group examining the links between the innate and the humoral immune systems and began to develop a strong interest in the role of novel molecular adjuvants such as C3d trimers or octamers in generating improved vaccination profiles and reagents.

Oct 2006-Dec 2008. Accepted a position as Lecturer in Medicine (nephrology) within the Institute of Human Genetics, Newcastle University where I developed an interest in the role of the immune system in atypical haemolytic ureamic syndrome and membranoproliferative glomerulonephritis.

Jan 2009 - 2019. Transferred within Newcastle University to the newly formed Institute of Cellular Medicine and the Applied Immunobiology grouping.

Nov 2019 - Member of the Translational and Clinical research Institute and the Immunity & Inflammation theme. Please see research for current ongoing projects.

Roles and Responsibilities

Group leader, research focus on the complement system, kidney and eye disease, vaccine adjuvants based on C3d, and the development of FH based therapeutics, see research.

Provide lectures and laboratory projects to students at Newcastle University  

Head of autoimmune aHUS research and analysis (NRCTC).

Chair - Immunology North East (British Society of Immunology regional group)

Co-Chair - Local organising committee for 29th International Complement Workshop

Board Member of European Complement Network

Qualifications

Doctor of Philosophy (Medical Biochemistry), UWCM, March 1997.

Diploma in Bio-Medical methods, UWCM, July 1994.

Bachelor of Science (2/1 hons) in Immunology, University of Glasgow, July 1993.

Previous Positions

2002-2006, Wellcome Trust RCD fellow & Lecturer, Cardiff University

2000-2002, Wellcome Trust Fellow, University of Wales College of Medicine

1999 –2000 NIH Research Fellow, University of Colorado Health sciences center

1997- 1999, Wellcome Trust Prize travelling Fellow, University of Colorado Health sciences center

Memberships

British Society for Immunology,

International Complement Society

European Complement Network

Renal Association

aHUS Rare disease group of RADAR

MPGN/DDD/C3G Rare disease group of RADAR

Honours and Award

2002 Research Career Development Fellowship, The Wellcome Trust

1997 Prize International Travelling Fellowship, The Wellcome Trust

1993 PhD scholarship, University of Wales College of Medicine

Informal Interests

Keen hiker, photographer, amateur artist and enjoy stimulating conversation, meeting new faces and as such have always enjoyed travel.

Google Scholar: Click here.

Research Interests

The complement system (Innate immunity), particularly Factor H, Factor H related proteins and C3 in both mouse and man.

Autoantibodies (in Kidney Diseases)

B cell development and the Humoral Immune response.C3d as a molecular adjuvant.

Other Expertise

Transgenic mice and certain Immune response models, Autoantibody ELISA (specifically anti Factor H ). Complement factor H related proteins. C3 Glomerulopathy (C3G) or Membranoproliferative glomerulonephritis (MPGN) and atypical Haemolytic ureamic syndrome (aHUS).

Current Work

>AutoAbs to Factor H . We have published several articles on the involvement of anti-Factor H autoantibodies in the development of aHUS and C3G.

>Understanding the function of complement factor H related proteins (FHRs) through the development of unique monoclonal antibodies and recombinant protein assays. We are working with Prof Mike Holers team in Denver Colorado to study the mouse FHRs and have recently investigated CFHL1 through funding from the NCKRF.

>Proof of concept funding looking at novel bacterial protein based adjuvants. C3d, a breakdown fragment of the complement protein C3, through its binding to complement receptor 2 has been shown to amplify the humoral immune response to antigen many fold. We are currently working on use of proteins designed to capture this function to aid vaccine delivery and potency we are also working with colleagues in Bath to improve vaccine transport. See our recent work in this area.

> anti-complement Therapeutics: Development of targeted factor H (FH) fusion proteins in order to protect the kidney from run away C activation. We are using existing (CR2-FH and Mini-FH) and novel strategies (homo-dimeric mini-FH) to target the C inhibitory domains of FH to the kidney (and the eye).

> C3 gain of function study: We have developed a unique C3 gain of function comparative model to investigate the mechanisms that pre-dispose to aHUS, the mechanisms that lead to pathology in the disease and to investigate treatment solutions, such as homo-dimeric mini-FH.

Pet project; examining the role of C3 and CR2 in the development of B cell tolerance

Future Research

The role of complement in the development of AMD (development of novel models/treatments)

The development of new models of aHUS investigating the trigger and role of the coagulation pathway. 

Research Roles

Lab based scientist, currently writing grants and papers. My Group currently consists of 1 technician, 3 PhDs (primary and co supervised), 4 PDRAs and 3 Clinical fellows

Esteem Indicators

Invited Tutor at the 16th European Meeting for Complement in Human Disease conference in Hungary september 2009.

Invited member of recently established MPGN (DDD) Rare Kidney diseases sub-group of the Renal association.

Founder member of Complement UK

Part of the International Complement Societies standardisation team with respect to development of a standard anti-FH autoantibody ELISA

Funding

MRC role of properdin and C7 in the development of aHUS; use of anti-complement drugs in C3 GOF mice. Testing of HDM-FH in ischemia reperfusion injury using Ex vivo Normothermic perfusion of pig kidney.

NCKRF and Newcastle Healthcare Charities supporting work surrounding the C3 gain of function model

Kidney Research UK supporting a PhD on trigger events in aHUS and Project grant looking at use of anti-complement drugs in complement mediated renal diseases

Patents Filed.

P031134GB "Modified Complement proteins and uses thereof"

P030973GB "Recombinant mature Complement Factor I"

1619965.5 "Immunogenic compositions comprising Sbi protein and use thereof" (with University of Bath)

Postgraduate Teaching

MRes research project supervision

MRes Lecturer, B cells

Undergraduate Teaching (B940/B900)

CMB3000/1 Undergraduate research project supervision

BMS3021 Lecture on complement in health and disease, provide seminar

BGM3039 Lecture on complement therapeutics

PSC2019 Lecture on Kidney Physiology

BMS2013 Presentation Marking

CMB2000 Ethics Essay Marking

• Articles

  • Brocklebank V, Walsh PR, Smith-Jackson K, Hallam TM, Marchbank KJ, Wilson V, Bigirumurame T, Dutt T, Montgomery EK, Malina M, Wong EKS, Johnson S, Sheerin NS, Kavanagh D. Atypical haemolytic uraemic syndrome in the era of terminal complement inhibition- An observational cohort study. Blood 2023, 142(16), 1371-1386.
  • Saha S, Rundle S, Kotsopoulos IC, Begbie J, Howarth R, Pappworth IY, Mukhopadhyay A, Kucukmetin A, Marchbank KJ, Curtin N. Determining the Potential of DNA Damage Response (DDR) Inhibitors in Cervical Cancer Therapy. Cancers 2022, 14(17), 4288.
  • Hallam TM, Cox TE, Smith-Jackson K, Brocklebank V, Baral AJ, Tzoumas N, Steel DH, Wong EKS, Shuttleworth VG, Lotery AJ, Harris CL, Marchbank KJ, Kavanagh D. A novel method for real-time analysis of the complement C3b:FH:FI complex reveals dominant negative CFI variants in Age-related Macular Degeneration. Frontiers in Immunology 2022, 13, 1028760.
  • McMahon O, Hallam TM, Patel S, Harris CL, Menny A, Zelek WM, Widjajahakim R, Java A, Tzoumas N, Steel DHW, Shuttleworth VG, Smith-Jackson K, Brocklebank V, Griffiths H, Cree AJ, Atkinson JP, Lotery AJ, Bubeck D, Morgan BP, Marchbank KJ, Seddon JM, Kavanagh D. The Rare C9 P167S Risk Variant for Age-related Macular Degeneration Increases Polymerization of the Terminal Component of the Complement Cascade. Human Molecular Genetics 2021, 30(13), 1188-1199.
  • Kerr H, Herbert AP, Makou E, Abramczyk D, Malik TH, Lomax-Browne H, Yang Y, Pappworth IY, Denton H, Richards A, Marchbank KJ, Pickering MC, Barlow PN. Murine Factor H Co-Produced in Yeast With Protein Disulfide Isomerase Ameliorated C3 Dysregulation in Factor H-Deficient Mice. Frontiers in Immunology 2021, 12, 681098.
  • Wahid AA, Dunphy RW, Macpherson A, Gibson BG, Kulik L, Whale K, Back C, Hallam TM, Alkhawaja B, Martin RL, Meschede I, Laabei M, Lawson ADG, Holers VM, Watts AG, Crennell SJ, Harris CL, Marchbank KJ, van den Elsen JMH. Insights Into the Structure-Function Relationships of Dimeric C3d Fragments. Frontiers in Immunology 2021, 12, 714055.
  • Kamala O, Malik TH, Hallam TM, Cox TE, Yang Y, Vyas F, Luli S, Connelly C, Gibson B, Smith-Jackson K, Denton H, Pappworth IY, Huang L, Kavanagh D, Pickering MC, Marchbank KJ. Homodimeric Minimal Factor H: In Vivo Tracking and Extended Dosing Studies in Factor H Deficient Mice. Frontiers in Immunology 2021, 12, 752916.
  • Malik TH, Gitterman DP, Lavin DP, Lomax-Browne HJ, Hiemeyer EC, Moran LB, Boroviak K, Cook HT, Gilmore AC, Mandwie M, Ahmad A, Alexander IE, Logan GJ, Marchbank KJ, Bradley A, Pickering MC. Gain-of-function factor H–related 5 protein impairs glomerular complement regulation resulting in kidney damage. Proceedings of the National Academy of Sciences of the United States of America 2021, 118(13), e2022722118.
  • Malik TH, Gitterman DP, Lavin DP, Lomax-Browne HJ, Hiemeyer EC, Moran LB, Boroviak K, Cook HT, Gilmore AC, Mandwie M, Ahmad A, Alexander IE, Logan GJ, Marchbank KJ, Bradley A, Pickering MC. Gain-of-function factor H-related 5 protein impairs glomerular complement regulation resulting in kidney damage. PNAS 2021, 118(13), e2022722118.
  • Wong EKS, Hallam T, Brocklebank V, Walsh P, Smith-Jackson K, Shuttleworth V, Cox T, Anderson H, Barlow PN, Marchbank KJ, Harris C, Kavanagh D. Functional characterisation of rare genetic variants in the N-terminus of complement factor H in aHUS, C3G and AMD. Frontiers in immunology 2021, 11, 602284.
  • Wong E, Marchbank K, Lomax-Browne H, Pappworth I, Denton H, Cooke K, Ward S, McLoughlin AC, Richardson G, Wilson V, Harris C, Morgan BP, Hakobyan S, McAlinden P, Gale D, Maxwell H, Christian M, Malcomson R, Goodship T, Marks S, Pickering M, Kavanagh D, Cook H, Johnson S. C3 Glomerulopathy and Related Disorders in Children. Clinical Journal of the American Society of Nephrology 2021, 16(11), 1639-1651.
  • Turro E, Astle WJ, Graf S, Greene D, Allen HL, Frontini M, Stephens J, Downes K, Tuna S, Deevi SVV, Aitman TJ, Bennett DL, Calleja P, Caulfield MJ, Chinnery PF, Dixon PH, James R, Laffan MA, Levine AP, Maher ER, Markus HS, Morales J, Morrell NW, Mumford AD, Rankin S, Rendon A, Richardson S, Roberts I, Roy NBA, Saleem MA, Smith KGC, Tan RYY, Thrasher AJ, Watkins H, Webster AR, Wilkins MR, Williamson C, Whitworth J, Humphray S, Abbs S, Abulhoul L, Adlard J, Ahmed M, Aitman TJ, Alachkar H, Allsup DJ, Almeida-King J, Ancliff P, Antrobus R, Armstrong R, Arno G, Ashford S, Astle WJ, Attwood A, Aurora P, Babbs C, Bacchelli C, Bakchoul T, Banka S, Bariana T, Barwell J, Batista J, Baxendale HE, Beales PL, Bennett DL, Bentley DR, Bentley DR, Bierzynska A, Biss T, Bitner-Glindzicz MAK, Black GC, Bleda M, Blesneac I, Bockenhauer D, Bogaard H, Bourne CJ, Boyce S, Bradley JR, Bragin E, Breen G, Brennan P, Brewer C, Brown M, Browning AC, Browning MJ, Buchan RJ, Buckland MS, Bueser T, Diz CB, Burn J, Burns SO, Burren OS, Burren OS, Burrows N, Calleja P, Campbell C, Carr-White G, Carss K, Carss K, Casey R, Caulfield MJ, Chambers J, Chambers J, Chan MMY, Cheah C, Cheng F, Chinnery PF, Chitre M, Christian MT, Church C, Clayton-Smith J, Cleary M, Brod NC, Coghlan G, Colby E, Cole TRP, Collins J, Collins PW, Colombo C, Compton CJ, Condliffe R, Cook S, Cook HT, Cooper N, Corris PA, Furnell A, Cunningham F, Curry NS, Cutler AJ, Daniels MJ, Dattani M, Daugherty LC, Davis J, De Soyza A, Deevi SVV, Dent T, Deshpande C, Dewhurst EF, Dixon PH, Douzgou S, Downes K, Drazyk AM, Drewe E, Duarte D, Dutt T, Edgar JDM, Edwards K, Egner W, Ekani MN, Elliott P, Erber WN, Erwood M, Estiu MC, Evans DG, Evans G, Everington T, Eyries M, Fassihi H, Favier R, Findhammer J, Fletcher D, Flinter FA, Floto RA, Fowler T, Fox J, Frary AJ, French CE, Freson K, Frontini M, Gale DP, Gale DP, Gall H, Ganesan V, Gattens M, Geoghegan C, Gerighty TSA, Gharavi AG, Ghio S, Ghofrani H-A, Gibbs JSR, Gibson K, Gilmour KC, Girerd B, Gleadall NS, Goddard S, Goldstein DB, Gomez K, Gordins P, Gosal D, Graf S, Graham J, Grassi L, Greene D, Greenhalgh L, Greinacher A, Gresele P, Griffiths P, Grigoriadou S, Grocock RJ, Grozeva D, Gurnell M, Hackett S, Hadinnapola C, Hague WM, Hague R, Haimel M, Haimel M, Hall M, Hanson HL, Haque E, Harkness K, Harper AR, Harris CL, Hart D, Hassan A, Hayman G, Henderson A, Herwadkar A, Hoffman J, Holden S, Horvath R, Houlden H, Houweling AC, Howard LS, Hu F, Hudson G, Hughes J, Huissoon AP, Humbert M, Humphray S, Hunter S, Hurles M, Irving M, Izatt L, James R, Johnson SA, Jolles S, Jolley J, Josifova D, Jurkute N, Karten T, Karten J, Kasanicki MA, Kazkaz H, Kazmi R, Kelleher P, Kelly AM, Kelsall W, Kempster C, Kiely DG, Kingston N, Klima R, Koelling N, Kostadima M, Kovacs G, Koziell A, Koziell A, Kreuzhuber R, Kuijpers TW, Kumar A, Kumararatne D, Kurian MA, Laffan MA, Lalloo F, Lambert M, Allen HL, Lawrie A, Layton DM, Lench N, Lentaigne C, Lester T, Levine AP, Linger R, Longhurst H, Lorenzo LE, Louka E, Lyons PA, Machado RD, MacKenzie Ross RV, Madan B, Maher ER, Maimaris J, Malka S, Mangles S, Mapeta R, Mapeta R, Marchbank KJ, Marks S, Markus HS, Marschall H-U, Marshall A, Martin J, Mathias M, Matthews E, Maxwell H, McAlinden P, McCarthy MI, McKinney H, McMahon A, Meacham S, Mead AJ, Castello IM, Megy K, Megy K, Mehta SG, Michaelides M, Millar C, Mohammed SN, Moledina S, Montani D, Moore AT, Morales J, Morrell NW, Mozere M, Muir KW, Mumford AD, Nemeth AH, Newman WG, Newnham M, Noorani S, Nurden P, O'Sullivan J, Obaji S, Odhams C, Okoli S, Olschewski A, Olschewski H, Ong KR, Oram SH, Ormondroyd E, Ormondroyd E, Ouwehand WH, Palles C, Papadia S, Park S-M, Parry D, Patel S, Paterson J, Peacock A, Pearce SH, Peden J, Peerlinck K, Pepke-Zaba J, Petersen R, Pilkington C, Poole KES, Prathalingam R, Psaila B, Pyle A, Quinton R, Rahman S, Rankin S, Rao A, Raymond FL, Rayner-Matthews PJ, Rees C, Rendon A, Renton T, Rhodes CJ, Rice ASC, Richardson S, Richter A, Robert L, Roberts I, Rogers A, Rose SJ, Ross-Russell R, Roughley C, Roy NBA, Ruddy DM, Sadeghi-Alavijeh O, Saleem MA, Samani N, Samarghitean C, Sanchis-Juan A, Sanchis-Juan A, Sargur RB, Sarkany RN, Satchell S, Savic S, Sayer JA, Sayer G, Scelsi L, Schaefer AM, Schulman S, Scott R, Scully M, Searle C, Seeger W, Sen A, Sewell WAC, Seyres D, Shah N, Shamardina O, Shamardina O, Shapiro SE, Shaw AC, Short PJ, Sibson K, Side L, Simeoni I, Simpson MA, Sims MC, Sivapalaratnam S, Smedley D, Smith KR, Smith KGC, Snape K, Soranzo N, Soubrier F, Southgate L, Spasic-Boskovic O, Staines S, Staples E, Stark H, Stark H, Stephens J, Steward C, Stirrups KE, Stuckey A, Suntharalingam J, Swietlik EM, Syrris P, Tait RC, Talks K, Tan RYY, Tate K, Taylor JM, Taylor JC, Thaventhiran JE, Themistocleous AC, Themistocleous AC, Thomas E, Thomas D, Thomas MJ, Thomas P, Thomson K, Thrasher AJ, Threadgold G, Thys C, Thys C, Tilly T, Tischkowitz M, Titterton C, Todd JA, Toh C-H, Tolhuis B, Tomlinson IP, Toshner M, Traylor M, Treacy C, Treadaway P, Trembath R, Tuna S, Turek W, Turro E, Twiss P, Vale T, Geet CV, Zuydam N, Vandekuilen M, Vandersteen AM, Vazquez-Lopez M, von Ziegenweidt J, Noordegraaf AV, Wagner A, Waisfisz Q, Walker SM, Walker N, Walter K, Ware JS, Watkins H, Watt C, Webster AR, Wedderburn L, Wei W, Welch SB, Wessels J, Westbury SK, Westwood J-P, Wharton J, Whitehorn D, Whitworth J, Wilkie AOM, Wilkins MR, Williamson C, Wilson BT, Wong EKS, Wood N, Wood Y, Woods CG, Woodward ER, Wort SJ, Worth A, Wright M, Yates K, Yong PFK, Young T, Yu P, Yu-Wai-Man P, Zlamalova E, Kingston N, Walker N, Bradley JR, Ashford S, Penkett CJ, Penkett CJ, Freson K, Stirrups KE, Raymond FL, Ouwehand WH. Whole-genome sequencing of patients with rare diseases in a national health system. Nature 2020, 583, 96-102.
  • Doekhie A, Slade MN, Cliff L, Weaver L, Castaing R, Paulin J, Chen Y-C, Edler KJ, Koumanov F, Marchbank KJ, van den Elsen JMH, Sartbaeva A. Thermal resilience of ensilicated lysozyme via calorimetric and in vivo analysis. RCS Advances 2020, 10(50), 29789-29796.
  • Nayagam JS, McGrath S, Montasser M, Delaney M, Cairns TD, Marchbank KJ, Yang Y, Denton H, Sacks SH, Cook HT, Shah S, Heaton N, Pickering MC, Suddle A. Successful simultaneous liver-kidney transplantation for renal failure associated with hereditary complement C3 deficiency. American Journal of Transplantation 2020, 20(8), 2260-2263.
  • Hallam TM, Marchbank KJ, Harris CL, Osmond C, Shuttleworth VG, Griffiths H, Cree AJ, Kavanagh D, Lotery AJ. Rare Genetic Variants in Complement Factor I Lead to Low FI Plasma Levels Resulting in Increased Risk of Age-Related Macular Degeneration. IOVS 2020, 61(6), 18.
  • Brocklebank V, Kumar G, Howie AJ, Chandar J, Milford DV, Craze J, Evans J, Finlay E, Freundlich M, Gale DP, Inward C, Mraz M, Jones C, Wong W, Marks SD, Connolly J, Corner BM, Smith-Jackson K, Walsh PR, Marchbank KJ, Harris C, Wilson V, Wong EKS, Malina M, Johnson S, Sheerin NS, Kavanagh D. Long-term outcomes and response to treatment in DGKE nephropathy. Kidney International 2020, 97(6), 1260-1274.
  • Levine AP, Chan MMY, Sadeghi-Alavijeh O, Wong EKS, Cook HT, Ashford S, Carss k, Christian MT, Hall M, Harris C, McAlinden P, Marchbank KJ, Marks SD, Maxwell H, Megy K, Penkett CJ, Mozere M, Stirrups KE, Tuna S, Wessels J, Whitehorn D, Johnson SA, Gale DP. Large scale whole-genome sequencing reveals the genetic architecture of primary membranoproliferative glomerulonephritis and C3 glomerulopathy. Journal of the American Society of Nephrology 2020, 31(1).
  • Doekhie A, Dattani R, Chen Y-C, Yang Y, Smith A, Silve AP, Koumanov F, Wells SA, Edler KJ, Marchbank KJ, van-den-Elsen JMH, Sartbaeva A. Ensilicated tetanus antigen retains immunogenicity: in vivo study and time-resolved SAXS characterization. Scientific Reports 2020, 10, 9243.
  • Yang Y, Back CR, Gräwert MA, Wahid AA, Denton H, Kildani R, Paulin J, Wörner K, Kaiser W, Svergun DI, Sartbaeva A, Watts AG, Marchbank KJ, vandenElsen JMH. Utilization of Staphylococcal Immune Evasion Protein Sbi as a Novel Vaccine Adjuvant. Frontiers in Immunology 2019, 9, 3139.
  • Smith-Jackson K, Yang Y, Denton H, Pappworth IY, Cooke K, Barlow PN, Atkinson JP, Liszewski MK, Pickering MC, Kavanagh D, Cook T, Marchbank KJ. Hyper-functional complement C3 promotes C5-dependent atypical hemolytic uremic syndrome in mice . Journal of Clinical Investigation 2019, 129(3), 1061-1075.
  • Wei W, Tuna S, Keogh MJ, Smith KR, Aitman TJ, Beales PL, Bennett DL, Gale DP, Bitner-Glindzicz MAK, Black GC, Brennan P, Elliott P, Flinter FA, Floto RA, Houlden H, Irving M, Koziell A, Maher ER, Markus HS, Morrell NW, Newman WG, Roberts I, Sayer JA, Smith KGC, Taylor JC, Watkins H, Webster AR, Wilkie AOM, Williamson C, NIHR BioResource - Rare Diseases, 100,000 Genomes Project - Rare Diseases Pilot, Ashford S, Penkett CJ, Stirrups KE, Rendon A, Ouwehand WH, Bradley JR, Raymond FL, Caulfield M, Turro E, Chinnery PF. Germline selection shapes human mitochondrial DNA diversity. Science 2019, 364(6442), eaau6520.
  • Antonioli AH, White J, Crawford F, Renner B, Marchbank KJ, Hannan JP, Thurman JM, Marrack P, Holers VM. Modulation of the Alternative Pathway of Complement by Murine Factor H-Related Proteins. Journal of Immunology 2018, 200(1), 316-326.
  • Yang Y, Denton H, Davies OR, Smith-Jackson K, Kerr H, Herbert A, Barlow PN, Pickering MC, Marchbank KJ. An Engineered Complement Factor H Construct for Treatment of C₃ Glomerulopathy. Journal of the American Society of Nephrology 2018, 29(6), 1649-1661.
  • He Y-G, Pappworth IY, Rossbach A, Paulin J, Mavimba T, Hayes C, Kulik L, Holers VM, Knight AM, Marchbank KJ. A novel C3d-containing oligomeric vaccine provides insight into the viability of testing human C3d-based vaccines in mice. Immunobiology 2018, 223(1), 125-134.
  • Challis RC, Ring T, Xu Y, Wong EKS, Flossmann O, Roberts ISD, Ahmed S, Wetherall M, Salkus G, Brocklebank B, Fester J, Strain L, Wilson V, Wood KM, Marchbank KJ, Santibanez-Koref M, Goodship THJ, Kavanagh D. Thrombotic Microangiopathy in Inverted Formin 2-Mediated Renal Disease. Journal of the American Society of Nephrology 2017, 28(4), 1084-1091.
  • Chen Y, Smith T, Hicks R, Doekhie A, Koumanov F, Wells S, Edler K, van-den-Elsen J, Holman G, Marchbank K, Sartbaeva A. Thermal stability, storage and release of proteins with tailored fit in silica. Scientific Reports 2017, 7, 46568.
  • Brocklebank V, Johnson S, Sheerin TP, Marks SD, Gilbert RD, Tyerman K, Kinoshita M, Awan A, Kaur A, Webb N, Hegde S, Finlay E, Fitzpatrick M, Walsh P, Wong EKS, Booth C, Kerecuk L, Salama A, Almond M, Inward C, Goodship TH, Sheerin N, Marchbank KJ, Kavanagh D. Factor H autoantibody is associated with atypical hemolytic uremic syndrome in children in the United Kingdom and Ireland. Kidney International 2017, 92(5), Pages 1261-1271.
  • Kerr H, Wong E, Makou E, Yang Y, Marchbank K, Kavanagh D, Richards A, Herbert A, Barlow P. Disease-linked mutations in factor H reveal pivotal role of cofactor activity in self surface-selective regulation of complement activation. Journal of Biological Chemistry 2017, 292, 13345-13360.
  • Phillips EH, Westwood JP, Brocklebank V, Wong EKS, Tellez JO, Marchbank KJ, McGuckin S, Gale DP, Connolly J, Goodship THJ, Kavanagh D, Scully MA. The role of ADAMTS-13 activity and complement mutational analysis in differentiating acute thrombotic microangiopathies. Journal of Thrombosis and Haemostasis 2016, 14(1), 175-185.
  • Schmidt CQ, Harder M, Nichols E, Hebecker M, Anliker M, Höchsmann B, Simmet T, Csincsi ÁI, Uzonyi B, Pappworth IY, Ricklin D, Lambris JD, Schrezenmeier H, Józsi M, Marchbank KJ. Selectivity of C3-opsonin targeted complement inhibitors: a distinct advantage in the protection of erythrocytes from paroxysmal nocturnal hemoglobinuria patients . Immunobiology 2016, 221(4), 503-511.
  • Challis RC, Araujo GSR, Wong EKS, Anderson HE, Awan A, Dorman AM, Waldron M, Wilson V, Brocklebank V, Strain L, Morgan BP, Harris CL, Marchbank KJ, Goodship THJ, Kavanagh D. A De Novo Deletion in the Regulators of Complement Activation Cluster Producing a Hybrid Complement Factor H/Complement Factor H–Related 3 Gene in Atypical Hemolytic Uremic Syndrome. Journal of the American Society of Nephrology 2016, 27(6), 1617-1624.
  • Watson R, Wearmouth E, McLoughlin A-C, Jackson A, Ward S, Bertram P, Bennaceur K, Barker CE, Pappworth IY, Kavanagh D, Lea SM, Atkinson JP, Goodship THJ, Marchbank KJ. Autoantibodies to CD59, CD55, CD46 or CD35 are not associated with atypical haemolytic uraemic syndrome (aHUS). Molecular Immunology 2015, 63(2), 287-296.
  • Nichols E, Barbour TD, Pappworth IY, Wong EKS, Palmer J, Sheerin N, Pickering MC, Marchbank KJ. An extended mini-complement factor H molecule ameliorates experimental C3 glomerulopathy. Kidney International 2015, 88(6), 1314-1322.
  • Nichols E, Jones R, Watson R, Pepper CJ, Fegan C, Marchbank KJ. A CD21 low phenotype, with no evidence of autoantibodies to complement proteins, is consistent with a poor prognosis in CLL. Oncotarget 2015, 6(32), 32669-32680.
  • Watson R, Lindner S, Bordereau P, Hunze EM, Tak F, Ngo S, Zipfel PF, Skerka C, Dragon-Durey MA, Marchbank KJ. Standardisation of the factor H autoantibody assay. Immunobiology 2014, 219(1), 9-16.
  • Wong EKS, Anderson HE, Herbert AP, Challis RC, Brown P, Reis GS, Tellez JO, Strain L, Fluck N, Humphrey A, Macleod A, Richards A, Ahlert D, Santibanez-Koref M, Barlow PN, Marchbank KJ, Harris CL, Goodship THJ, Kavanagh D. Characterization of a Factor H Mutation That Perturbs the Alternative Pathway of Complement in a Family with Membranoproliferative GN. Journal of American Society of Nephrology 2014, 25(11), 2425-2433.
  • Blom AM, Volokhina EB, Fransson V, Stromberg P, Berghard L, Viktorelius M, Mollnes TE, Lopez-Trascasa M, van den Heuvel LP, Goodship TH, Marchbank KJ, Okroj M. A novel method for direct measurement of complement convertases activity in human serum. Clinical & Experimental Immunology 2014, 178(1), 142-153.
  • Wilson V, Darlay R, Wong W, Wood KM, McFarlane J, Schejbel L, Schmidt IM, Harris CL, Tellez J, Hunze EM, Marchbank K, Goodship JA, Goodship THJ. Genotype/Phenotype Correlations in Complement Factor H Deficiency Arising From Uniparental Isodisomy. American Journal of Kidney Diseases 2013, 62(5), 978-983.
  • Holmes LV, Strain L, Staniforth SJ, Moore I, Marchbank K, Kavanagh D, Goodship JA, Cordell HJ, Goodship THJ. Determining the Population Frequency of the CFHR3/CFHR1 Deletion at 1q32. PLoS ONE 2013, 8(4), e60352.
  • Brown JH, Tellez J, Wilson V, Mackie IJ, Scully M, Tredger MM, Moore I, McDougall NI, Strain L, Marchbank KJ, Sheerin NS, O'Grady J, Harris CL, Goodship THJ. Postpartum aHUS Secondary to a Genetic Abnormality in Factor H Acquired Through Liver Transplantation. American Journal of Transplantation 2012, 12(6), 1632-1636.
  • Kavanagh D, Pappworth IY, Anderson H, Hayes CM, Moore I, Hunze EM, Bennaceur K, Roversi P, Lea S, Strain L, Ward R, Plant N, Nailescu C, Goodship TH, Marchbank KJ. Factor I Autoantibodies in Patients with Atypical Hemolytic Uremic Syndrome: Disease-Associated or an Epiphenomenon?. Clinical Journal of the American Society of Nephrology 2012, 7(3), 417-426.
  • Goodship THJ, Pappworth IY, Toth T, Denton M, Houlberg K, McCormick F, Warland D, Moore I, Hunze EM, Staniforth SJ, Hayes C, Cavalcante DP, Kavanagh D, Strain L, Herbert AP, Schmidt CQ, Barlow PN, Harris CL, Marchbank KJ. Factor H autoantibodies in membranoproliferative glomerulonephritis. Molecular Immunology 2012, 52(3-4), 200-206.
  • Francis NJ, McNicholas B, Awan A, Waldron M, Reddan D, Sadlier D, Kavanagh D, Strain L, Marchbank KJ, Harris CL, Goodship THJ. A novel hybrid CFH/CFHR3 gene generated by a microhomology-mediated deletion in familial atypical hemolytic uremic syndrome. Blood 2012, 119(2), 591-601.
  • Dhillon B, Wright AF, Tufail A, Pappworth I, Hayward C, Moore I, Strain L, Kavanagh D, Barlow PN, Herbert AP, Schmidt CQ, Armbrecht AM, Laude A, Deary IJ, Staniforth SJ, Holmes LV, Goodship THJ, Marchbank KJ. Complement Factor H Autoantibodies and Age-Related Macular Degeneration. Investigative Ophthalmology & Visual Science 2010, 51(11), 5858-5863.
  • Moore I, Strain L, Pappworth I, Kavanagh D, Barlow PN, Herbert AP, Schmidt CQ, Staniforth SJ, Holmes LV, Ward R, Morgan L, Goodship THJ, Marchbank KJ. Association of factor H autoantibodies with deletions of CFHR1, CFHR3, CFHR4, and with mutations in CFH, CFI, CD46, and C3 in patients with atypical hemolytic uremic syndrome. Blood 2010, 115(2), 379-387.
  • Depoil D, Fleire S, Treanor BL, Weber M, Harwood NE, Marchbank KL, Tybulewicz VLJ, Batista FD. CD19 is essential for B cell activation by promoting B cell receptor-antigen microcluster formation in response to membrane-bound ligand. Nature Immunology 2008, 9(1), 63-72.
  • Kulik L, Marchbank KJ, Lyubchenko T, Kuhn KA, Liubchenko GA, Haluszczak C, Gipson MG, Boackle SA, Holers VM. Intrinsic B cell hypo-responsiveness in mice prematurely expressing human CR2/CD21 during B cell development. European Journal of Immunology 2007, 37(3), 623-633.
  • Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ. Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21) transgenic mice is partially recovered in the absence of C3. Molecular Immunology 2007, 44(13), 3434-3444.
  • Birrell L, Kulik L, Morgan BP, Holers VM, Marchbank KJ. B Cells from Mice Prematurely Expressing Human Complement Receptor Type 2 are Unresponsive to T-Dependent Antigens. Journal of Immunology 2005, 174(11), 6974-6982.

• Conference Proceedings (inc. Abstracts)

  • Marchbank KJ, Kavanagh D, Harris CL. Editorial. In: 29th International Complement Workshop. 2023, Elsevier GmbH.
  • Saha S, Howarth R, Pappworth I, Marchbank K, Curtin N. Potential use of the PARP inhibitor rucaparib to enhance cervical cancer treatment. In: 32nd EORTC-NCI-AACR Symposium on Molecular Targets and Cancer Therapeutics. 2020, Virtual: Elsevier Ltd.
  • Saha S, Howarth R, Dubrovska A, Marchbank K, Curtin N. Increasing the therapeutic index of radiation by combination with rucaparib in cervical cancer. In: ESTRO 2020 Online Congress. 2020, Online: Elsevier.
  • Saha S, Howarth R, Pappworth I, Gentles L, Marchbank K, Curtin N. Exploring rucaparib as chemo-radiosensitiser to increase therapeutic ratio in cervical cancer. In: DNA Damage Responses and Cancer: Innovations from Radiobiology to Radiotherapy conference. 2020, Virtual: European Association for Cancer Research.
  • Smith-Jackson K, Denton H, Cook K, Pickering MC, Cook TH, Marchbank KJ. A novel C3 gain of function mouse model of atypical haemolytic uraemic syndrome. In: 26th International Complement Workshop (XXVI ICW). 2016, Kanazawa, Japan: Elsevier GmbH - Urban und Fischer.
  • Wong EKS, Marchbank K, Pappworth I, Walters R, Lomax-Browne H, Harris C, Morgan P, Pickering M, Goodship THJ, Malcomson R, Cook T, Johnson S, MPGN C3G Rare Dis Grp. The national study of membranoproliferative glomerulonephrtis and C3 glomerulopathy - characterisation of the paediatric cohort. In: 52nd ERA-EDTA Congress. 2015, London, UK: Oxford University Press.
  • Nichols EM, Barbour TD, Kerr H, Pappworth IY, Wong EKS, Palmer J, Sheerin NS, Herbert A, Barlow PN, Pickering MC, Marchbank KJ. FH1-5^(18-20) ameliorates experimental C3 glomerulopathy; generation and testing of a murine version for further pre-clinical experiments. In: 15th European Meeting on Complement in Human Disease 2015. 2015, Uppsala, Sweden: Elsevier.

• Editorials

  • Marchbank KJ, Frazer-Abel A, Dragon-Durey M-A, Dixon BP. Editorial: Autoantibodies in Kidney Diseases. Frontiers in Immunology 2020, 11, 591338.
  • Smith-Jackson K, Marchbank KJ. Targeting properdin in the treatment of atypical haemolytic uraemic syndrome: better than eculizumab?. Annals of Translational Medicine 2019, 6(Suppl 1), S62.

• Note

  • Lango Allen H, Lango Allen H, Rae W, Greene D, Staples E, Zhang Z, Rivers E, Penkett CJ, Stephens J, Deevi SVV, Thomas MJ, Brown M, Tuijnenburg P, Worth A, Hanson S, Linger RJ, Buckland MS, Gilmour KC, Seneviratne SL, Sansom DM, Lynch AG, Ellinghaus E, Ellinghaus D, Jorgensen SF, Karlsen TH, Stirrups KE, Cutler AJ, Kumararatne DS, Chandra A, Edgar JDM, Cooper N, Huissoon AP, Goddard S, Jolles S, Schuetz C, Boschann F, Abbs S, Adhya Z, Adlard J, Afzal M, Ahmed I, Ahmed M, Ahmed S, Aitman TJ, Alachkar H, Alamelu J, Alikhan R, Allen CE, Allen L, Allsup DJ, Alvi A, Ambegaonkar G, Anantharachagan A, Ancliff P, Anderson J, Antrobus R, Armstrong R, Arno G, Arumugakani G, Arya R, Ashford S, Astle WJ, Attwood A, Austin S, Aydinok Y, Ayub W, Babbs C, Bacchelli C, Baglin T, Bakchoul T, Bariana TK, Barratt J, Barwell J, Baski J, Bates RW, Batista J, Baxendale HE, Baxendale HE, Baynam G, Bennett DL, Bethune C, Bhatnagar N, Bibi S, Bierzynska A, Biss T, Bitner-Glindzicz MAK, Bleda M, Blesneac I, Boardman B, Boddana P, Bogaard HJ, Booth C, Boyce S, Bradley JR, Brady A, Breen G, Brennan P, Brewer C, Briley A, Brown M, Brown R, Browning MJ, Brownlie M, Bryson CJ, Buchan RJ, Buck J, Buckland MS, Bueser T, Diz CB, Burns SO, Burren OS, Burren OS, Calleja P, Carmichael J, Carr-White G, Carss KJ, Casey R, Chalmers E, Chambers J, Chambers J, Chan MMY, Chan MV, Chandra A, Cheng F, Chinn IK, Chinnery PF, Chitre M, Chong S, Christian MT, Church C, Clement EM, Brod NC, Clifford H, Clowes VE, Coghlan G, Colby E, Cole TRP, Collins JH, Collins PW, Condliffe R, Cook HT, Cook S, Cookson V, Cooper N, Corris PA, Creaser-Myers A, Crisp-Hihn A, Curry NS, Cutler AJ, Da Costa R, Danesino C, Daniels MJ, Darby D, Daugherty LC, Davies EG, Davies S, Davis J, de Bree GJ, Deacock S, Deegan PB, Deevi SVV, Dempster J, Dent T, Deshpande C, Devlin LA, Dewhurst EF, Dixit AK, Dixon PH, Doffinger R, Dolling H, Dormand N, Downes K, Drazyk AM, Drewe E, Duarte D, Dutt T, Edgar JDM, Edwards KE, Egner W, Ekani MN, El-Shanawany T, Elkhalifa S, Elston T, Emmerson I, Erber WN, Erwood M, Estiu MC, Evans DG, Evans G, Everington T, Eyries M, Farmery JHR, Farmery JHR, Favier R, Firth HV, Fitzpatrick MM, Fletcher D, Flinter FA, Fox JC, Frary AJ, French CE, Freson K, Frontini M, Furie B, Gale DP, Gall HJ, Gardham A, Gaspar HB, Gattens M, Ghali N, Ghataorhe PK, Ghio S, Ghofrani H-A, Ghurye R, Gibbs JSR, Gilbert RD, Gilmour KC, Girerd B, Girling JC, Gissen P, Gleadall NS, Gleadall NS, Goddard S, Gordins P, Gordins P, Gorman KM, Gosal D, Graf S, Grassi L, Greene D, Greenhalgh AJ, Greenhalgh L, Greinacher A, Gresele P, Griffiths PG, Griffiths S, Grigoriadou S, Grigoriadou S, Grozeva D, Hackett SJ, Hadden RDM, Hadinnapola C, Hague R, Hague WM, Haimel M, Hall M, Halmagyi C, Hammerton T, Hanson HL, Harkness K, Harper AR, Harper L, Harris C, Harrison C, Hart D, Hassan A, Hayman G, Heemskerk JWM, Hegde S, Henderson A, Henderson RH, Hensiek A, Henskens YMC, Herwadkar A, Herwadkar A, Hodgson J, Hoffman J, Holden S, Holder M, Horvath R, Houlden H, Houweling AC, Howard LS, Hu F, Hudson G, Hughes S, Hughes S, Huis in 't Veld AE, Huissoon AP, Humbert M, Hurles ME, Hurst JA, Irvine V, Izatt L, James R, Jeevaratnam P, Johnson M, Johnson SA, Jolles S, Jolley JD, Jones B, Jones J, Josifova D, Jurkute N, Karim YM, Karoshi MA, Kasanicki MA, Kazkaz H, Kazmi R, Keeling D, Kelleher P, Kelly AM, Kempster C, Kennedy F, Kiani S, Kiely DG, Kingston N, Kinsey S, Klein N, Klima R, Knox E, Kostadima MA, Kovacs G, Koziell AB, Kreuzhuber R, Krishnakumar D, Kuijpers TW, Kumar A, Kumararatne DS, Kurian MA, Laffan J, Laffan MA, Lalloo F, Lambert MP, Lawman SHA, Lawrie A, Layton DM, Lear SE, Lees MM, Lentaigne C, Levine AP, Lewington AJP, Li W, Liesner R, Linger RJ, Liu B, Longhurst H, Lorenzo LE, Louka E, Hadeler SL, Lyons PA, Macdougall M, Machado RD, MacKenzie Ross RV, Mackillop LH, MacLaren R, Madan B, Magee L, Mahdi-Rogers M, Maher ER, Maimaris J, Maimaris J, Makris M, Mangles S, Manson A, Manzur A, Mapeta R, Marchbank KJ, Mark PB, Marks S, Markus HS, Marschall H-U, Marshall A, Martin JM, Masati L, Mathias M, Matser V, Matthews EL, Maw A, Maxwell H, McAlinden P, McCarthy MI, McDermott EM, McGowan SJ, McJannet C, McKinney H, Meacham S, Mead AJ, Castello IM, Meehan S, Megy K, Megy K, Mehta S, Mercer CL, Michaelides M, Michell AC, Milford D, Millar CM, Millar H, Mistry A, Moenen F, Moledina S, Montani D, Moore AT, Moore J, Morrell NW, Morrisson V, Mozere M, Muir KW, Mumford AD, Murng SHK, Nasir I, Nejentsev S, Newnham M, Ng J, Ngoh A, Noorani S, Noori M, Nurden P, O'Sullivan JM, Obaji S, Okoli S, Oksenhendler E, Olschewski A, Olschewski H, Ong ACM, Ong KR, Oram H, Ormondroyd E, Othman S, Ouwehand WH, Pantazis A, Papadia S, Papandreou A, Park S-M, Parker APJ, Parry D, Parsons G, Pasi KJ, Paterson J, Payne JH, Peacock AJ, Peerlinck K, Penkett CJ, Pepke-Zaba J, Perry D, Petersen R, Piechowski-Jozwiak B, Pinto F, Polwarth GJ, Ponsford MJ, Prasad S, Prokopenko I, Psaila B, Pyle A, Qasim W, Quinn E, Quinti I, Raina S, Ranganathan L, Rankin J, Rankin S, Rao A, Raymond FL, Rayner-Matthews PJ, Rayner-Matthews PJ, Rehnstrom K, Reid E, Reilly MM, Renton T, Revel-Vilk S, Rhodes CJ, Rice ASC, Richards EE, Richards M, Richardson S, Richter A, Robert L, Roberts I, Rondina MT, Rosser E, Rothwell P, Roughley C, Roy NB, Rue-Albrecht K, Sadeghi-Alavijeh O, Saleem MA, Salmon RM, Samani NJ, Samarghitean C, Samarghitean C, Sambrook JG, Sanchis-Juan A, Sanchis-Juan A, Sandford R, Santra S, Sargur RB, Sargur RB, Satchell SC, Savic S, Scelsi L, Schotte G, Schulman S, Schulze H, Scott R, Scully M, Searle C, Seeger W, Seneviratne SL, Sewell WAC, Seyres D, Shackley F, Shamardina O, Shapiro SE, Sharma P, Shehata HA, Shipley D, Shtoyerman R, Sibson K, Side L, Simeoni I, Simeoni I, Simpson M, Sims MC, Sinha MD, Sivapalaratnam S, Skytte A-B, Smith KGC, Snape K, Sneddon L, Sohal A, Soubrier F, Southgate L, Southwood M, Splitt M, Staines S, Staples E, Stark H, Stauss H, Steele CL, Stein D, Stein PE, Stephens J, Stirrups KE, Stock S, Stubbs MJ, Suntharalingam J, Swietlik EM, Symington E, Tait RC, Talks K, Tan RYY, Taylor GB, Thachil J, Thaventhiran JED, Thaventhiran JED, Themistocleous AC, Thomas DC, Thomas E, Thomas MJ, Thomas P, Thompson DA, Thomson K, Thrasher AJ, Thys C, Tilly T, Tischkowitz M, Titterton C, Todd JA, Toh C-H, Tool ATJ, Toshner MR, Traylor M, Treacy CM, Treadaway P, Trembath RC, Trippier S, Tuna S, Turek W, Turro E, Upton PD, Urniaz R, Vale T, Van Geet C, van Zuydam N, Vandersteen AM, Vazquez-Lopez M, Veltman MWM, Vogt J, von Ziegenweidt J, Noordegraaf AV, Vora A, Vries MJA, Wakeling EL, Walker N, Walker SM, Walsh R, Wanjiku I, Ware JS, Warner TQ, Wassmer E, Watkins H, Watson HG, Watt C, Waugh D, Webb N, Webster AR, Wei W, Welch A, Welch SB, Werring D, Wessels J, Westbury SK, Westwood J-PW, Wharton J, Whitehorn D, Whitworth J, Wilkins MR, Willcocks L, Williams DJ, Williamson C, Wong EKS, Wood N, Wood Y, Woods CG, Woodward ER, Workman S, Wort SJ, Worth A, Yates K, Yeatman N, Yong PFK, Young T, Yu P, Yu-Wai-Man P, Zlamalova E, Lyons PA, Hurles ME, Savic S, Burns SO, Kuijpers TW, Turro E, Ouwehand WH, Thrasher AJ, Smith KGC. Publisher Correction: Whole-genome sequencing of a sporadic primary immunodeficiency cohort (Nature, (2020), 583, 7814, (90-95), 10.1038/s41586-020-2265-1). Nature 2020, 584, E2.

• Review

  • Gibson BG, Cox TE, Marchbank KJ. Contribution of animal models to the mechanistic understanding of Alternative Pathway and Amplification Loop (AP/AL)-driven Complement-mediated Diseases. Immunological Reviews 2023, 313(1), 194-216.