Staff Profile

Qualifications:

1993                          Dr. in Human Medicine, University of Medicine and Pharmacy Gr. T. Popa, Iasi, Romania

1994                          Dr. Med. Univ., Faculty of Medicine, Leopold Franzens University, Innsbruck, Austria

1998                          Diploma of General Physician

                                  Austrian Chamber of Physicians, Vienna, Austria

2004                          PhD in Molecular Biology, University of Manchester, UK

Career:

1995-1999                 Junior medical doctor at the General Hospital of Lienz, Austria

1999-2000                 General Practitioner in Austria

2000-2004                 PhD student (DNA damage, checkpoints and telomeres)                     

                                  Prof. David Lydall group, University of Manchester, UK

2001-2003                 Scientific Associate, Intercytex LTD (Stem cell research)

                                  Manchester, UK (part time during my PhD).   

2004-2006                 Postdoctoral Research Associate

                                  Prof. David Lydall group, IAH, Newcastle University

Oct 2006-April 2007    Lecturer in Genome Instability, IAH, Newcastle University

May 2007-April 2013  Wellcome Trust Career Development Fellow, IAH, Newcastle 

                                    University

May 2013-present       Lecturer in Genome Instability, Newcastle University  

Scientific interests:

My group studies factors and mechanisms that are involved in DNA damage responses and the maintenance of genomic stability. We have identified the first DNA polymerase that targets dysfunctional telomeres and contributes to the maintenance of cellular senescence and the first protein (Rif1) able to inhibit the recruitment of checkpoint proteins and Replication Protein A (RPA) to DNA damage. Moreover, we have demonstrated that cells are able to repair long single stranded DNA lesions in conjunction with osmotic pressure and the inositol pathway. Earlier discoveries include the unusual mechanism that permits eukaryotic cells to proliferate indefinitely after losing telomeres (at a time when such idea was considered absurd) and the identification of the first DNA nuclease degrading telomeres in eukaryotic cells.

BGM3063 Biochemistry of Gene Expression: 1 lecture

MMB8011 Biology of Ageing: 1 lecture

MMB8008 Chromosome Biology and Cell Cycle Control in Health and Disease: 1 lecture

BGM2056 DNA Replication, recombination and Repair: Practical and Q&A

BGM3061 Genetic variation in common disease: 1 lecture

BGM1004 Genetics: 3x2 seminars

BMS3010 Genetics and Human Disease: 1 lecture

BGM3058 Integrated Genetics: 6 lectures

BMS2014 The Biology of Ageing: 2 lectures

Module leader for BGM2056 and BGM3058

• Articles

  • Robertson CM, Xue Y, Chowdhury S, Maringele L. A CDK-Dependent Phosphorylation of a Novel Domain of Rif1 Regulates its Function during Telomere Damage and Other Types of Stress. Molecular and Cellular Biology 2023, 43(5), 185-199.
  • Raykov V, Marvin ME, Louis EJ, Maringele L. Telomere Dysfunction Triggers Palindrome Formation Independently of Double-Strand Break Repair Mechanisms. Genetics 2016, 203(4), 1659-1668.
  • Xue Y, Marvin ME, Ivanova IG, Lydall D, Louis EJ, Maringele L. Rif1 and Exo1 regulate the genomic instability following telomere losses. Aging Cell 2016, 15(3), 553-562.
  • Ivanova I, Maringele L. Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt. Nucleic Acids Research 2016, 44(8), 3728-3738.
  • Addinall SG, Holstein EM, Lawless C, Yu M, Chapman K, Banks AP, Ngo HP, Maringele L, Taschuk M, Young A, Ciesiolka A, Lister AL, Wipat A, Wilkinson DJ, Lydall D. Quantitative Fitness Analysis Shows That NMD Proteins and Many Other Protein Complexes Suppress or Enhance Distinct Telomere Cap Defects. PLoS Genetics 2011, 7(4), e1001362.
  • Deshpande AM, Ivanova IG, Raykov V, Xue Y, Maringele L. Polymerase Epsilon Is Required To Maintain Replicative Senescence. Molecular and Cellular Biology 2011, 31(8), 1637-1645.
  • Xue Y, Rushton MD, Maringele L. A Novel Checkpoint and RPA Inhibitory Pathway Regulated by Rif1. PLoS Genetics 2011, 7(12), e1002417.
  • LeBel C, Rosonina E, Sealey DCF, Pryde F, Lydall D, Maringele L, Harrington LA. Telomere Maintenance and Survival in Saccharomyces cerevisiae in the Absence of Telomerase and RAD52. Genetics 2009, 182(3), 671-684.
  • Zubko MK, Maringele L, Foster SS, Lydall D. Detecting repair intermediates in vivo: Effects of DNA damage response genes on single-stranded DNA accumulation at uncapped telomeres in budding yeast. Methods in Enzymology 2006, 409, 285-300.
  • Downey M, Houlsworth R, Maringele L, Rollie A, Brehme M, Galicia S, Guillard S, Partington M, Zubko MK, Krogan NJ, Emili A, Greenblatt JF, Harrington L, Lydall D, Durocher D. A Genome-Wide Screen Identifies the Evolutionarily Conserved KEOPS Complex as a Telomere Regulator. Cell 2006, 124(6), 1155-1168.
  • Maringele L, Lydall D. The PAL-mechanism of Chromosome Maintenance: Causes and Consequences. Cell Cycle 2005, 4(6), 747-751.
  • Maringele L, Lydall D. Telomerase- and recombination-independent immortalization of budding yeast. Genes and Development 2004, 18(21), 2663-2675.
  • Maringele L, Lydall D. EXO1 Plays a Role in Generating Type I and Type II Survivors in Budding Yeast. Genetics 2004, 166(4), 1641-1649.
  • Maringele L, Lydall D. EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Δ mutants. Genes & Development 2002, 16(15), 1919-1933.

• Book Chapter

  • Maringele L, Lydall D. Pulsed Field Gel Electrophoresis of Budding Yeast Chromosomes. In: Xiao, W, ed. Yeast Protocols. Totawa, New Jersey: Humana Press, 2005, pp.65-74.