Serial Block Face Imaging
Create 3D reconstructions at the ultrastructural level.
Thanks to a successful BBSRC bid we can now offer serial block face imaging with the Zeiss Sigma Scanning Electron Microscope (FEGSEM) incorporating the Gatan 3view. (SBF-SEM)
Data from SBF-SEM can be organised into a stack of images. By segmenting out an area of interest, in each image in the stack, you can then generate a 3D model.
Specialist software can be used to display and edit the 3D representation of your data.
Software available to 3view users:
- Amira
- Blender
- MIB
- IMOD
- ImageJ
- Digital Micrograph
In EMRS we offer training on 3D reconstruction and biological image analysis and the related software.
