Publications

Using data generated within FCCF

In a publication that includes data (main figures or supplemental) generated within the FCCF, please state:

“We acknowledge the Newcastle University Flow Cytometry Core Facility (FCCF) for assistance with the generation of Flow Cytometry data”

Working with FCCF team members

If a member of the FCCF team has worked closely with you on a project, you should acknowledge this.

It is good practice to include their name in the acknowledgement section at least.

In situations where they have made substantial intellectual input, you can offer them a co-authorship. This should be discussed at the earliest opportunity.

Using our logo

You can use our logo in a talk or a poster for a conference. This is intended to be for acknowledgement purposes. Download our logo below 

Paragraph for grant support

“All the Flow Cytometry outlined in this grant proposal will be conducted using systems that are located in the Newcastle University Flow Cytometry Core Facility (FCCF). The instruments are maintained to a very high standard by a team of specialists led by Dr Andrew Filby. Dr Filby is an expert in cytometry applications and method development with over 15 years’ experience in the field. He is also recognised by the International Society for the Advancement of Cytometry (ISAC) as an “Emerging Leader” in the core facility network. Particular attention is paid by Dr Filby and his team to minimising/eliminating sources of potentially confounding measurement error through tracking of instrument performance and the use of the correct biological controls. Furthermore the facility will train and support users in all aspects of cytometry including panel design, sample preparation, sample acquisition, data analysis and data interpretation. The facility also actively encourages adherence to the “MIFlowCyte” minimum information standards about flow cytometry experimentation”.

Regulation and Role of αE Integrin and Gut Homing Integrins in Migration and Retention of Intestinal Lymphocytes during Inflammatory Bowel Disease.
Keir ME, Fuh F, Ichikawa R, Acres M, Hackney JA, Hulme G, Carey CD, Palmer J, Jones CJ, Long AK, Jiang J, Klabunde S, Mansfield JC, Looney CM, Faubion WA, Filby A, Kirby JA, McBride J and Lamb CA
J Immunol November 1, 2021, 207 (9) 2245-2254; DOI: https://doi.org/10.4049/jimmunol.2100220

Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans.
Cytlak U, Resteu A, Pagan S, Green K, Milne P, Maisuria S, McDonald D, Hulme G, Filby A, Carpenter B, Queen R, Hambleton S, Hague R, Lango Allen H, Thaventhiran JED, Doody G, Collin M, Bigley V. Immunity. 2020 Aug 18;53(2):353-370.e8. doi: 10.1016/j.immuni.2020.07.003. Epub 2020 Jul 30. PMID: 32735845 Free PMC article.

Telomerase Activation to Reverse Immunosenescence in Elderly Patients With Acute Coronary Syndrome: Protocol for a Randomized Pilot Trial.
Maier R, Bawamia B, Bennaceur K, Dunn S, Marsay L, Amoah R, Kasim A, Filby A, Austin D, Hancock H, Spyridopoulos I. JMIR Res Protoc. 2020 Sep 23;9(9):e19456. doi: 10.2196/19456. PMID: 32965237 Free PMC article.

Modifying Regulatory Practices to Create a Safe and Effective Working Environment Within a Shared Resource Laboratory During a Global Pandemic.
Filby A, Haviland DL, Jones DD, López AB, Orlowski-Oliver E, Rieger AM. Cytometry A. 2020 Nov 15:10.1002/cyto.a.24264. doi: 10.1002/cyto.a.24264. Online ahead of print. PMID: 33190383 Free PMC article. No abstract available

Biosafety during a pandemic: shared resource laboratories rise to the challenge.
Aspland AM, Douagi I, Filby A, Jellison ER, Martinez L, Shinko D, Smith AL, Tang VA, Thornton S. Cytometry A. 2020 Dec 2:10.1002/cyto.a.24280. doi: 10.1002/cyto.a.24280. Online ahead of print. PMID: 33289290 Free PMC article.

Developmental cell programs are co-opted in inflammatory skin disease.
Reynolds G, Vegh P, Fletcher J, Poyner EFM, Stephenson E, Goh I, Botting RA, Huang N, Olabi B, Dubois A, Dixon D, Green K, Maunder D, Engelbert J, Efremova M, Polański K, Jardine L, Jones C, Ness T, Horsfall D, McGrath J, Carey C, Popescu DM, Webb S, Wang XN, Sayer B, Park JE, Negri VA, Belokhvostova D, Lynch MD, McDonald D, Filby A, Hagai T, Meyer KB, Husain A, Coxhead J, Vento-Tormo R, Behjati S, Lisgo S, Villani AC, Bacardit J, Jones PH, O'Toole EA, Ogg GS, Rajan N, Reynolds NJ, Teichmann SA, Watt FM, Haniffa M. Science. 2021 Jan 22;371(6527):eaba6500. doi: 10.1126/science.aba6500. PMID: 33479125

A Cytometrist's Guide to Coordinating and Performing Effective COVID‐19 Research
Chattopadhyay PK , Filby A 2, Jellison ER, Ferrari G ,Green C 5, Cherian S 6, Irish J 7, Virginia Litwin V, other members of the ISAC COVID‐19 Work Group
Cytometry A. 2020 Aug 16;10.1002/cyto.a.24210. doi: 10.1002/cyto.a.24210. Online ahead of print.

Decoding mitochondrial heterogeneity in single muscle fibres by imaging mass cytometry
Warren C, McDonald D, Capaldi R, Deehan D, Taylor RW, Filby A, Turnbull DM, Lawless C, Vincent AE.
Scientific Reports,2020 Sep 18;10(1):15336. doi: 10.1038/s41598-020-70885-3.

A cell atlas of human thymic development defines T cell repertoire formation.
Park JE, Botting RA, Domínguez Conde C, Popescu DM, Lavaert M, Kunz DJ, Goh I, Stephenson E, Ragazzini R, Tuck E, Wilbrey-Clark A, Roberts K, Kedlian VR, Ferdinand JR, He X, Webb S, Maunder D, Vandamme N, Mahbubani KT, Polanski K, Mamanova L, Bolt L, Crossland D, de Rita F, Fuller A, Filby A, Reynolds G, Dixon D, Saeb-Parsy K, Lisgo S, Henderson D, Vento-Tormo R, Bayraktar OA, Barker RA, Meyer KB, Saeys Y, Bonfanti P, Behjati S, Clatworthy MR, Taghon T, Haniffa M, Teichmann SA.
Science. 2020 Feb 21;367(6480). pii: eaay3224. doi: 10.1126/science.aay3224.

Classification of Human White Blood Cells Using Machine Learning for Stain‐Free Imaging Flow Cytometry
Lippeveld M, Knill C, Ladlow E, Fuller A, Michaelis LJ, Saeys Y, Filby A, Peralta D
Cytometry Part A November 2019 doi.org/10.1002/cyto.a.23920

Decoding human fetal liver haematopoiesis
Popescu D-M, Botting RA, Stephenson E, Green K, Webb S, Jardine L, Calderbank EF, Polanski K, Goh I, Efremova M, Acres M, Maunder D, Vegh P, Gitton Y, Park J-E, Vento-Tormo R, Miao Z, Dixon D, Rowell R, McDonald D, Fletcher J, Poyner E, Reynolds G, Mather M, Moldovan C, Mamanova L, Greig F, Young MD, Meyer KB, Lisgo S, Bacardit J, Fuller A, Millar B, Innes B, Lindsay S, Stubbington MJT, Kowalczyk MS, Li B, Ashenberg O, Tabaka M, Dionne D, Tickle TL, Slyper M, Rozenblatt-Rosen O, Filby A, Carey P, Villani AC, Roy A, Regev A, Chédotal A, Roberts I, Göttgens B, Behjati S, Laurenti E, Teichmann SA, Haniffa M
Nature 199 October 2019 doi.org/10.1038/s41586-019-1652-y

Spatiotemporal immune zonation of the human kidney
Stewart BJ, Ferdinand JR, Young MD, Mitchell TJ, Loudon KW, Riding AM, Richoz N, Frazer GL, Staniforth JUL, Vieira Braga FA, Botting RA, Popescu DM, Vento-Tormo R, Stephenson E, Cagan A, Farndon SJ, Polanski K, Efremova M, Green K, Velasco-Herrera MDC, Guzzo C, Collord G, Mamanova L, Aho T, Armitage JN, Riddick ACP, Mushtaq I, Farrell S, Rampling D, Nicholson J, Filby A, Burge J, Lisgo S, Lindsay S, Bajenoff M, Warren AY, Stewart GD, Sebire N, Coleman N, Haniffa M, Teichmann SA, Behjati S, Clatworthy MR
Science 27 Sep 2019: Vol. 365, Issue 6460, pp. 1461-1466
DOI: 10.1126/science.aat5031

Human interleukin-2 receptor β mutations associated with defects in immunity and peripheral tolerance.
Zhang Z, Gothe F, Pennamen P, James JR, McDonald D, Mata CP, Modis Y, Alazami AM, Acres M, Haller W, Bowen C, Döffinger R, Sinclair J, Brothers S, Zhang Y, Matthews HF, Naudion S, Pelluard F, Alajlan H, Yamazaki Y, Notarangelo LD, Thaventhiran JE, Engelhardt KR, Al-Mousa H, Hambleton S, Rooryck C, Smith KGC, Lenardo MJ.
J Exp Med. 2019 Apr 30. pii: jem.20182304. doi: 10.1084/jem.20182304. [Epub ahead of print]

Lipopolysaccharide inhalation recruits monocytes and dendritic cell subsets to the alveolar airspace.
Jardine L, Wiscombe S, Reynolds G, McDonald D, Fuller A, Green K, Filby A, Forrest I, Ruchaud-Sparagano MH, Scott J, Collin M, Haniffa M, Simpson AJ.
Nat Commun. 2019 Apr 30;10(1):1999. doi: 10.1038/s41467-019-09913-4.

Shared Resource Laboratory (SRL) Communications-A New Journal Type
Davies D, Filby A, Lannigan J
Cytometry A. 2018 Dec 14. doi: 10.1002/cyto.a.23703. [Epub ahead of print]

Single-cell reconstruction of the early maternal–fetal interface in humans 
Vento-Tormo R, Efremova M, Botting RA, Turco MY, Vento-Tormo M, Meyer KB, Park JE, Stephenson E, Polański K, Goncalves A, Gardner L, Holmqvist S, Henriksson J, Zou A, Sharkey AM, Millar B, Innes B, Wood L, Wilbrey-Clark A, Payne RP, Ivarsson MA, Lisgo S, Filby A, Rowitch DH, Bulmer JN, Wright GJ, Stubbington MJT, Haniffa M, Moffett A, Teichmann SA.
Nature. 2018 Nov;563(7731):347-353. doi: 10.1038/s41586-018-0698-6. Epub 2018 Nov 14.

Early PREdiction of sepsis using leukocyte surface biomarkers: the ExPRES-sepsis cohort study.
Shankar-Hari M, Datta D, Wilson J, Assi V, Stephen J, Weir CJ, Rennie J, Antonelli J, Bateman A, Felton JM, Warner N, Judge K, Keenan J, Wang A, Burpee T, Brown AK, Lewis SM, Mare T, Roy AI, Wright J, Hulme G, Dimmick I, Gray A, Rossi AG, Simpson AJ, Conway Morris A, Walsh TS
Intensive Care Med. 2018 Oct 5. doi: 10.1007/s00134-018-5389-0

Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors.
Young MD, Mitchell TJ, Vieira Braga FA, Tran MGB, Stewart BJ, Ferdinand JR, Collord G, Botting RA, Popescu DM, Loudon KW, Vento-Tormo R, Stephenson E, Cagan A, Farndon SJ, Del Castillo Velasco-Herrera M, Guzzo C, Richoz N, Mamanova L, Aho T, Armitage JN, Riddick ACP, Mushtaq I, Farrell S, Rampling D, Nicholson J, Filby A, Burge J, Lisgo S, Maxwell PH, Lindsay S1, Warren AY, Stewart GD, Sebire N, Coleman N, Haniffa M, Teichmann SA, Clatworthy M, Behjati S

Science. 2018 Aug 10;361(6402):594-599. doi: 10.1126/science.aat1699.

Cell-surface signatures of immune dysfunction risk-stratify critically ill patients: INFECT study
Morris A.C., Datta D, Shankar-Hari M, Stephen J, Weir C.J., Rennie J, Antonelli J, Bateman A, Warner N, Judge K, Keenan J, Wang A, BurpeeK T, Brown A, Lewis S. M, Mare T, Roy A.I, Hulme G, Dimmick I, Rossi A.G, Simpson A.J, Walsh T.S
Intensive Care Med 2018 June doi.org/10.1007/s00134-018-5247-0

Global phenotypic characterisation of human platelet lysate expanded MSCs by high-throughput flow cytometry.
Reis M, McDonald D, Nicholson L, Godthardt K, Knobel S, Dickinson AM, Filby A, Wang XN.
Sci Rep. 2018 Mar 2;8(1):3907. doi: 10.1038/s41598-018-22326-5.

Diagnostic Potential of Imaging Flow Cytometry
Doan M, Vorobjev I, Rees P, Filby A, Wolkenhauer O, Goldfeld A.E, Lieberman J, Barteneva N, Carpenter A.E, Hennig H,
Trends in Biotech. 2018 Jan 31. DOI: 10.1016/j.tibtech.2017.12.008

Guidelines for the use of flow cytometry and cell sorting in immunological studies
Cossarizza A, Chang H, Radbruch A, Andrä I, Annunziato F, Bacher P, Barnaba V, Battistini L, Bauer W. M, Baumgart S, Becher B, Beisker W, Berek C, Blanco A, Borsellino G, Boulais P.E., Brinkman R.R, Büscher M, Busch D.H., Bushnell T.P., Cao X, Cavani A, Chattopadhyay P. K., Cheng Q, Chow S, Clerici M, Cooke A, Cosma A, Cosmi L, Cumano A, Dang V. D., Davies D, De Biasi S, Del Zotto G, Della Bella S, Dellabona P, Deniz G, Dessing M, Diefenbach A, Di Santo j, Dieli F, Dolf A, Donnenberg V. S., Dörner T, Ehrhardt G. R. A., Endl E, Engel P, Engelhardt B, Esser C, Everts B, Falk C. S., Fehniger T. A., Filby A, et al
Eur. J. Immunol. 2017 October 11. 47: 1584–1797 DOI: 10.1002/eji.201646632 

A human patient-derived cellular model of Joubert syndrome reveals ciliary defects which can be rescued with targeted therapies
Srivastava S, Ramsbottom S.A., Molinari E, Alkanderi S, Filby A, White K, Henry C, Saunier S, Miles C.G, Sayer J.A
Hum Mol Genet. 2017 Sep 11. doi: 10.1093/hmg/ddx34 PMID 28973549 

Imaging cytometry: Automated morphology and feature extraction
Filby A, Houston J.P.
Cytometry A. 2017 Sep;91(9):851-853. doi: 10.1002/cyto.a.23200 
PMID:28940699

Reconstructing cell cycle and disease progression using deep learning
Eulenberg P, Köhler N, Blasi T, Filby A, Carpenter A.E, Rees P, Theis F.J, Wolf F.A.
Nat Commun. 2017 Sept 6, 8:463 doi:10.1038/s41467-017-00623-3

Cellular senescence drives age-dependent hepatic steatosis.
Ogrodnik M, Miwa S, Tchkonia T, Tiniakos D, Wilson CL, Lahat A, Day CP, Burt A, Palmer A, Anstee QM, Grellscheid SN, Hoeijmakers JHJ, Barnhoorn S, Mann DA, Bird TG, Vermeij WP, Kirkland JL, Passos JF, von Zglinicki T, Jurk D

Nat Commun. 2017 Jun 13;8:15691. doi: 10.1038/ncomms15691. PMID: 28608850

Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells

Borger JG, Morrison VL, Filby A, Garcia C, Uotila LM, Simbari F, Fagerholm SC, Zamoyska R.
J Immunol. 2017 Jun 21. pii: ji1700431. doi: 10.4049/jimmunol.1700431 PMID 28637901

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors.
Villani AC, Satija R, Reynolds G, Sarkizova S, Shekhar K, Fletcher J, Griesbeck M, Butler A, Zheng S, Lazo S, Jardine L, Dixon D, Stephenson E, Nilsson E, Grundberg I, McDonald D, Filby A, Li W, De Jager PL, Rozenblatt-Rosen O, Lane A, Haniffa M, Regev A, Hacohen N
Science. 2017 Apr 21;356(6335) pii: eaah4573. doi: 10.1126/science.aah4573 PMID: 28428369 

Sample preparation for flow cytometry benefits from some lateral thinking.
Filby A.
Cytometry. 2016 Nov 22. doi:10.1002/cyto.a.23021

An open-source solution for advanced imaging flow cytometry data analysis using machine learning.
Hennig H, Rees P, Blasi T, Kamentsky L, Hung J, Dao D, Carpenter AE, Filby A.
Methods. 2016 Sep 1. pii: S1046-2023(16)30291-2. doi: 10.1016/j.ymeth.2016.08.018. [Epub ahead of print] PMID 27594698

Early PREdiction of Severe Sepsis (ExPRES-Sepsis) study: protocol for an observational derivation study to discover potential leucocyte cell surface biomarkers.
Datta D, Conway Morris A, Antonelli J, Warner N, Brown KA, Wright J, Simpson AJ, Rennie J, Hulme G, Lewis SM, Mare TA, Cookson S, Weir CJ, Dimmick I, Keenan J, Rossi AG, Shankar-Hari M, Walsh TS; ExPRES Sepsis Investigators
BMJ Open. 2016 Aug 1;6(8):e011335. doi: 10.1136/bmjopen-2016-011335.PMID 27481622

Predictive value of cell-surface markers in infections in critically ill patients: protocol for an observational study (ImmuNe FailurE in Critical Therapy (INFECT) Study).
Conway Morris A, Datta D, Shankar-Hari M, Weir CJ, Rennie J, Antonelli J, Rossi AG, Warner N, Keenan J, Wang A, Brown KA, Lewis S, Mare T, Simpson AJ, Hulme G, Dimmick I, Walsh TS.
BMJ Open. 2016 Jul 18;6(7):e011326. doi: 10.1136/bmjopen-2016-011326.

Long-term in vitro maintenance of clonal abundance and leukaemia-initiating potential in acute lymphoblastic leukaemia.
Pal D, Blair HJ, Elder A, Dormon K, Rennie KJ, Coleman DJ, Weiland J, Rankin KS, Filby A, Heidenreich O, Vormoor J 
Leukemia. 2016 Apr 25. doi: 10.1038/leu.2016.79. [Epub ahead of print]
PMID: 27109511

Frequency and dynamics of leukemia-initiating cells during short-term ex vivo culture informs outcomes in acute myeloid leukemia patients.
Griessinger E, Anjos-Afonso F, Vargaftig J, Taussig D, Lassailly F, Prebet T, Imbert V, Nebout M, Vey N,Chabannon C, Filby A, Bollet-Quivogne F, Gribben JG, Peyron J, Bonnet D.
Cancer Res. 2016 Mar 9. pii: canres.2063.2015. [Epub ahead of print]
PubMed PMID: 26960976

Stepwise B-cell-dependent expansion of T helper clonotypes diversifies the T-cell response
Merkenschlager J, Ploquin MJ, Eksmond U, Andargachew R, Thorborn G, Filby A, Pepper M, Evavold B, Kassiotis G
Nat Commun. 2016 Jan 5;7:10281. doi: 
10.1038/ncomms10281. PubMed PMID: 26728651.

Label-free cell cycle analysis for high-throughput imaging flow cytometry
Blasi T, Hennig H, Summers HD, Theis FJ, Cerveira J, Patterson JO, Davies D, Filby A, Carpenter AE, Rees P
Nat Commun. 2016 Jan 7;7:10256. doi: 10.1038/ncomms10256
PubMed PMID: 26739115

The Analysis of Cell Cycle, Proliferation and Asymmetric Cell Division by Imaging Flow Cytometry
Andrew Filby, William Day, Sukhveer Purewal and Nuria Martinez-Martin
Chapter 5 in Imaging Flow Cytometry: Methods and Protocols. Methods in Molecular Biology, 2016.

New, clear fusion from the cellular perspective: Measuring cellular fusion in Saccharomyces cerevisiae by flow cytometry
Filby A.
Cytometry A. 2015 Jun 26.
doi: 10.1002/cyto.a.22714. [Epub ahead of print] PubMed PMID: 26115228.

An imaging flow cytometry-based approach to measuring the spatiotemporal calcium mobilisation in activated T cells.
Cerveira J, Begum J, Di Marco Barros R, van der Veen AG, Filby A.
J Immunol Methods. 2015 Aug;423:120-130. doi: 
10.1016/j.jim.2015.04.030. Epub 2015 May 9. PubMed PMID: 25967946.

An Imaging Flow Cytometry-based approach to analyse the fission yeast cell cycle in fixed cells. 
Patterson JO, Swaffer M, Filby A. 
Methods. 2015 Jul
1;82:74-84. doi: 10.1016/j.ymeth.2015.04.026. Epub 2015 May 4. PubMed PMID: 25952947.

Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution. 
Filby A, Begum J, Jalal M, Day W. 
Methods. 2015 Jul 1;82:29-37. doi: 10.1016/j.ymeth.2015.02.016.
Epub 2015 Mar 20. PubMed PMID: 25802116.

Dual proteolytic pathways govern glycolysis and immune competence.
Lu W, Zhang Y, McDonald DO, Jing H, Carroll B, Robertson N, Zhang Q, Griffin H, Sanderson S, Lakey JH, Morgan NV, Reynard LN, Zheng L, Murdock HM, Turvey SE, Hackett SJ, Prestidge T, Hall JM, Cant AJ, Matthews HF, Koref MF, Simon AK, Korolchuk VI, Lenardo MJ, Hambleton S, Su HC.
Cell. 2014 Dec 18;159(7):1578-90. doi: 10.1016/j.cell.2014.12.001.PMID: 25525876 [PubMed - indexed for MEDLINE]

"Mega" cytometry for a "mega" challenging cell type.
Filby A. 
Cytometry A. 2014 Apr;85(4):289-91. doi: 10.1002/cyto.a.22435. Epub 2014 Jan 16. PubMed PMID: 24436289.

A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution. 
Begum J, Day W, Henderson C, Purewal S, Cerveira J, Summers H, Rees P, Davies D, Filby A.
Cytometry A. 2013 Dec;83(12):1085-95. doi: 10.1002/cyto.a.22403. Epub 2013 Oct 25. PubMed PMID: 24166880.

Genetic tracing via DNGR-1 expression history defines dendritic cells as a hematopoietic lineage. 
Schraml BU, van Blijswijk J, Zelenay S, Whitney PG, Filby A, Acton SE, Rogers 
NC, Moncaut N, Carvajal JJ, Reis e Sousa C. 
Cell. 2013 Aug
15;154(4):843-58. doi: 10.1016/j.cell.2013.07.014. PubMed PMID: 23953115.

Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity.
Hawkins ED, Oliaro J, Kallies A, Belz GT, Filby A, Hogan T, Haynes N, Ramsbottom KM, Van Ham V, Kinwell T, Seddon B, Davies D, Tarlinton D, Lew AM, Humbert PO, Russell SM. 
Nat Commun. 2013;4:1801. doi:
10.1038/ncomms2796. PubMed PMID: 23653213.

Differential polarization of C-terminal Src kinase between naive and antigen-experienced CD8+ T cells.
Borger JG, Filby A, Zamoyska R. 
J Immunol. 2013 Apr 1;190(7):3089-99. doi: 10.4049/jimmunol.1202408. Epub 2013 Feb 20. PubMed PMID: 23427257.

Reporting imaging flow cytometry data for publication: why mask the detail?
Filby A, Davies D.
Cytometry A. 2012 Aug;81(8):637-42. doi: 10.1002/cyto.a.22091.
Epub 2012 Jul 3. PubMed PMID: 22760920.

Asymmetric segregation of polarized antigen on B cell division shapes presentation capacity.
Thaunat O, Granja AG, Barral P, Filby A, Montaner B, Collinson L, Martinez-Martin N, Harwood NE, Bruckbauer A, Batista FD.
Science.2012 Jan 27;335(6067):475-9. doi: 10.1126/science.1214100. PubMed PMID: 22282815.

Exome sequencing identifies GATA-2 mutation as the cause of dendritic cell, monocyte, B and NK lymphoid deficiency.
Dickinson RE, Griffin H, Bigley V, Reynard LN, Hussain R, Haniffa M, Lakey JH, Rahman T, Wang XN, McGovern N, Pagan S, Cookson S, McDonald D, Chua I, Wallis J, Cant A, Wright M, Keavney B, Chinnery PF, Loughlin J, Hambleton S, Santibanez-Koref M, Collin M.
Blood. 2011 Sep 8;118(10):2656-8. doi: 10.1182/blood-2011-06-360313. Epub 2011 Jul 15.
PMID: 21765025

An imaging flow cytometric method for measuring cell division history and molecular symmetry during mitosis.
Filby A, Perucha E, Summers H, Rees P, Chana P, Heck S, Lord GM, Davies D. 
Cytometry A. 2011 Jul;79(7):496-506. doi:
10.1002/cyto.a.21091. Epub 2011 Jun 2. PubMed PMID: 21638766.

IRF8 mutations and human dendritic-cell immunodeficiency.
Hambleton S, Salem S, Bustamante J, Bigley V, Boisson-Dupuis S, Azevedo J, Fortin A, Haniffa M, Ceron-Gutierrez L, Bacon CM, Menon G, Trouillet C, McDonald D, Carey P, Ginhoux F, Alsina L, Zumwalt TJ, Kong XF, Kumararatne D, Butler K, Hubeau M, Feinberg J, Al-Muhsen S, Cant A, Abel L, Chaussabel D, Doffinger R, Talesnik E, Grumach A, Duarte A, Abarca K, Moraes-Vasconcelos D, Burk D, Berghuis A, Geissmann F, Collin M, Casanova JL, Gros P.
N Engl J Med. 2011 Jul 14;365(2):127-38. doi: 10.1056/NEJMoa1100066. Epub 2011 Apr 27.
PMID: 21524210

Heterogeneous sensitivity of human acute myeloid leukemia to β-catenin down-modulation. 
Gandillet A, Park S, Lassailly F, Griessinger E, Vargaftig J, Filby A, Lister TA, Bonnet D.
Leukemia. 2011 May;25(5):770-80. doi:
10.1038/leu.2011.17. Epub 2011 Feb 22. PubMed PMID: 21339756; PubMed Central
PMCID: PMC4289854.

Mutation in the TCRα subunit constant gene (TRAC) leads to a human immunodeficiency disorder characterized by a lack of TCRαβ+ T cells.
Morgan NV, Goddard S, Cardno TS, McDonald D, Rahman F, Barge D, Ciupek A, Straatman-Iwanowska A, Pasha S, Guckian M, Anderson G, Huissoon A, Cant A, Tate WP, Hambleton S, Maher ER.
J Clin Invest. 2011 Feb;121(2):695-702. doi: 10.1172/JCI41931. Epub 2011 Jan 4.
PMID: 21206088

Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. 
Salmond RJ, Filby A, Pirinen N, Magee AI, Zamoyska R. 
Blood.2011 Jan 6;117(1):108-17. doi: 10.1182/blood-2010-03-277160. Epub 2010 Sep 28.
PubMed PMID: 20876849.

Whole-exome-sequencing-based discovery of human FADD deficiency.
Bolze A, Byun M, McDonald D, Morgan NV, Abhyankar A, Premkumar L, Puel A, Bacon CM, Rieux-Laucat F, Pang K, Britland A, Abel L, Cant A, Maher ER, Riedl SJ, Hambleton S, Casanova JL.
Am J Hum Genet. 2010 Dec 10;87(6):873-81. doi: 10.1016/j.ajhg.2010.10.028. Epub 2010 Nov 25.
PMID: 21109225

Vento-Tormo R1,2, Efremova M1, Botting RA3, Turco MY2,4,5, Vento-Tormo M6, Meyer KB1, Park JE1, Stephenson E3, Polański K1, Goncalves A1,7, Gardner L2,4, Holmqvist S8, Henriksson J1, Zou A1, Sharkey AM2,4, Millar B3, Innes B3, Wood L1, Wilbrey-Clark A1, Payne RP3, Ivarsson MA4, Lisgo S9, Filby A3, Rowitch DH8, Bulmer JN3, Wright GJ1, Stubbington MJT1, Haniffa M10,11,12, Moffett A13,14, Teichmann SA15,16,17.

Race between retroviral spread and CD4+ T-cell response determines the outcome of acute Friend virus infection.
Pike R, Filby A, Ploquin MJ, Eksmond U, Marques R, Antunes I, Hasenkrug K, Kassiotis G. 
J Virol. 2009 Nov;83(21):11211-22.doi: 10.1128/JVI.01225-09. Epub 2009 Aug 19. PubMed PMID: 19692462; PubMed
Central PMCID: PMC2772778.

Thyroid peroxidase forms thionamide-sensitive homodimers: relevance for immunomodulation of thyroid autoimmunity.
McDonald DO, Pearce SH.
J Mol Med (Berl). 2009 Oct;87(10):971-80. doi: 10.1007/s00109-009-0511-y. Epub 2009 Aug 8.
PMID: 19669106

T-cell receptor proximal signaling via the Src-family kinases, Lck and Fyn, influences T-cell activation, differentiation, and tolerance.
Salmond RJ, Filby A, Qureshi I, Caserta S, Zamoyska R. 
Immunol Rev. 2009 Mar;228(1):9-22.doi: 10.1111/j.1600-065X.2008.00745.x. Review. PubMed PMID: 19290918.

The tryptophan 620 allele of the lymphoid tyrosine phosphatase (PTPN22) gene predisposes to autoimmune Addison's disease.
Roycroft M, Fichna M, McDonald D, Owen K, Zurawek M, Gryczyńska M, Januszkiewicz-Lewandowska D, Fichna P, Cordell H, Donaldson P, Nowak J, Pearce S.
Clin Endocrinol (Oxf). 2009 Mar;70(3):358-62. doi: 10.1111/j.1365-2265.2008.03380.x. Epub 2008 Aug 15.
PMID: 18710467

Polymorphisms in CLEC16A and CIITA at 16p13 are associated with primary adrenal insufficiency.
Skinningsrud B, Husebye ES, Pearce SH, McDonald DO, Brandal K, Wolff AB, Løvås K, Egeland T, Undlien DE.
J Clin Endocrinol Metab. 2008 Sep;93(9):3310-7. doi: 10.1210/jc.2008-0821. Epub 2008 Jul 1.
PMID: 18593762

Fyn regulates the duration of TCR engagement needed for commitment to effector function.
Filby A, Seddon B, Kleczkowska J, Salmond R, Tomlinson P, Smida M, Lindquist JA, Schraven B, Zamoyska R. 
J Immunol. 2007 Oct 1;179(7):4635-44. PubMed PMID: 17878361.

Lck regulates the threshold of activation in primary T cells, while both Lck and Fyn contribute to the magnitude of the extracellular signal-related kinase response.
Lovatt M, Filby A, Parravicini V, Werlen G, Palmer E, Zamoyska R. 
Mol Cell Biol. 2006 Nov;26(22):8655-65. Epub 2006 Sep 11. PubMed PMID: 16966372; 
PubMed Central PMCID: PMC1636771.

The influence of the src-family kinases, Lck and Fyn, on T cell differentiation, survival and activation.
Zamoyska R, Basson A, Filby A, Legname G, Lovatt M, Seddon B.
Immunol Rev. 2003 Feb;191:107-18. Review. PubMed PMID: 12614355.

Interleukins 5 and 13 characterize immune responses to respiratory sensitizing acid anhydrides
Dearman RJ, Filby A, Humphreys IR, Kimber I.
J Appl Toxicol. 2002 Sep-Oct;22(5):317-25. PubMed PMID: 12355561.