Background

Qualifications

PhD in Biophysics, University of East Anglia

BSc Hons in Zoology, University of Liverpool

Previ ous Positions

1985-1987 SERC/NATO Fellow Centre de Biophysique Moleculaire, CNR Orleans France.

1987-90 EMBO/EMBL fellow European Molecular Biology Laboratory, Heidelberg. Germany

1990-1993 Staff Scientist, European Molecular Biology Laboratory, Heidelberg. Germany

1993-1997 Lecturer, School of Biochemistry and Genetics. Newcastle.

1997-2000 Reader, School of Biochemistry and Genetics. Newcastle.

2000- 2019 Professor of Structural Biochemistry, Institute for Cell and Molecular Biosciences

2019- Professor of Structural Biochemistry, Biosciences Institute

Research

Research Interests

We are interested in the engineering, structures, interactions and conformational changes of proteins in solution and in membranes. We use a combination of biochemical and biophysical tools but try to avoid the worst perils of reductionism (see footnote) by preserving a strong biological interest.

The core subject area is the bacterial outer membrane (a nanoscale Hadrian’s Wall) but this has spawned projects in natively unfolded proteins (the relaxed guys), bionanotechnology of surfaces (a chance to use physics tricks invented by others), neutron science (a chance to play on seriously big bits of kit) and biopharmaceutical analysis (doing less crazy experiments that are useful to industry at the same time).

In recent years we have also developed the unbelievably well behaved Caf1 protein polymer into a range of biomaterials.

We particularly enjoy new challenges in biophysical chemistry if they also take us to exotic, gastronomic or scenic locations.

If you are interested in our work please read some of our exciting, stunning publications and/or get in touch!

Our work has led to the formation of Newcastle Universiyt Spin Out Company MarraBIo (www.Marrabio.com)

Keywords: Biophysics, Membrane proteins, Protein-protein interactions, Bionanotechnology, Caf1, Protein Engineering

Postgraduate Supervision

Yes

Funding

BBSRC

Wellcome Trust

Industry

Footnote

"My own scientific career was a descent from higher to lower dimension, led by a desire to understand life. I went from animals to cells to bacteria, from bacteria to molecules, from molecules to electrons.

The story had its irony, for molecules and electrons have no life at all.

On my way, the life I was trying to study ran out between my fingers."

"The Perils of Reductionism" (1972) Albert Szent-Gyorgi

Teaching

Undergraduate Teaching

I have retired from undergraduate teaching

Postgraduate Teaching

I supervise PhD students

Publications

• Articles

  • Robson T, Shah DSH, Welbourn RJL, Phillips SR, Clifton LA, Lakey JH. Fully Aqueous Self-Assembly of a Gold-Nanoparticle-Based Pathogen Sensor. International Journal of Molecular Sciences 2023, 24(8), 7599.
  • Dura G, Crespo-Cuadrado M, Waller H, Peters DT, Ferreira-Duarte A, Lakey JH, Fulton DA. Exploiting Meltable Protein Hydrogels to Encapsulate and Culture Cells in 3D. Macromolecular Bioscience 2022, 22(9), 2200134.
  • Pirc K, Clifton LA, Yilmaz N, Saltalamacchia A, Mally M, Snoj T, Znidarsic N, Srnko M, Borisek J, Parkkila P, Albert I, Podobnik M, Numata K, Nurnberger T, Viitala T, Derganc J, Magistrato A, Lakey JH, Anderluh G. An oomycete NLP cytolysin forms transient small pores in lipid membranes. Science Advances 2022, 8(10), eabj9406.
  • Solovyova AS, Peters DT, Dura G, Waller H, Lakey JH, Fulton DA. Probing the oligomeric re-assembling of bacterial fimbriae in vitro: a small-angle X-ray scattering and analytical ultracentrifugation study. European Biophysics Journal 2021, 50, 597-611.
  • Dura G, Crespo-Cuadrado M, Waller H, Peters DT, Ferreira AM, Lakey JH, Fulton DA. Hydrogels of engineered bacterial fimbriae can finely tune 2D human cell culture. Biomaterials Science 2021, 9(7), 2542-2552.
  • Paracini N, Clifton LA, Lakey JH. Studying the surfaces of bacteria using neutron scattering: finding new openings for antibiotics. Biochemical Society transactions 2020, 48(5), 2139-2149.
  • Dominguez-Medina CC, Perez-Toledo M, Schager AE, Marshall JL, Cook CN, Bobat S, Hwang H, Chun BJ, Logan E, Bryant JA, Channell WM, Morris FC, Jossi SE, Alshayea A, Rossiter AE, Barrow PA, Horsnell WG, MacLennan CA, Henderson IR, Lakey JH, Gumbart JC, Lopez-Macias C, Bavro VN, Cunningham AF. Outer membrane protein size and LPS O-antigen define protective antibody targeting to the Salmonella surface. Nature communications 2020, 11(1), 851.
  • Dura G, Peters DT, Waller H, Yemm AI, Perkins ND, Ferreira AM, Crespo-Cuadrado M, Lakey JH, Fulton DA. A thermally reformable protein polymer. Chem 2020, 6(11), 3132-3151.
  • Clifton LA, Paracini N, Hughes AV, Lakey JH, Steinke N-J, Cooper JFK, Gavutis M, Skoda MWA. Self-Assembled Fluid Phase Floating Membranes with Tunable Water Interlayers. Langmuir 2019, 35(42), 13735-13744.
  • Lakey JH. Recent advances in neutron reflectivity studies of biological membranes. Current Opinions in Colloid and Interface Science 2019, 42, 33-40.
  • Al-Jawdah AD, Ivanova IG, Waller H, Perkins ND, Lakey JH, Peters DT. Induction of the immunoprotective coat of Yersinia pestis at body temperature is mediated by the Caf1R transcription factor. BMC Microbiology 2019, 19(1), 68.
  • Huang Y, Soliakov A, Le Brun AP, Macdonald C, Johnson CL, Solovyova AS, Waller H, Moore GR, Lakey JH. Helix N-Cap Residues Drive the Acid Unfolding That Is Essential in the Action of the Toxin Colicin A. Biochemistry 2019, 58(48), 4882-4892.
  • Peters DT, Waller H, Birch MA, Lakey JH. Engineered mosaic protein polymers; a simple route to multifunctional biomaterials. Journal of Biological Engineering 2019, 13, 54.
  • Dura G, Waller H, Gentile P, Lakey JH, Fulton DA. Tuneable hydrogels of Caf1 protein fibers. Materials Science and Engineering C 2018, 93, 88-95.
  • Robson T, Shah DSH, Solovyova AS, Lakey JH. Modular Protein Engineering Approach to the Functionalization of Gold Nanoparticles for Use in Clinical Diagnostics. ACS Applied Nano Materials 2018, 1(7), 3590-3599.
  • Paracini N, Clifton LA, Skoda MWA, Lakey JH. Liquid crystalline bacterial outer membranes are critical for antibiotic susceptibility. Proceedings of the National Academy of Sciences of the United States of America 2018, 115(32), E7587-E7594.
  • Akin S, Ulusu Y, Waller H, Lakey JH, Sonmezoglu S. Insight into Interface Engineering at TiO₂/Dye through Molecularly Functionalized Caf1 Biopolymer. ACS Sustainable Chemistry and Engineering 2018, 6(2), 1825-1836.
  • Peters DT, Kay L, Eswaran J, Lakey JH, Soundararajan M. Human Miro Proteins Act as NTP Hydrolases through a Novel, Non-Canonical Catalytic Mechanism. International Journal of Molecular Sciences 2018, 19(12), 3839.
  • Hwang H, Paracini N, Parks JM, Lakey JH, Gumbart JC. Distribution of mechanical stress in the Escherichia coli cell envelope. Biochimica et Biophysica Acta - Biomembranes 2018, 1860(12), 2566-2575.
  • Arunmanee W, Heenanc RK, Lakey JH. Determining the amphipol distribution within membrane-protein fibre samples using small-angle neutron scattering. Acta Crystallographica Section D 2018, 74(12), 1192-1199.
  • Ulusu Y, Dura G, Waller H, Benning MJ, Fulton DA, Lakey JH, Peters DH. Thermal stability and rheological properties of the ‘non-stick’ Caf1 biomaterial. Biomedical Materials 2017, 12(5), 051001.
  • Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH. The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophysical Journal 2017, 113(8), 1673-1684.
  • Harris JR, Soliakov A, Watkinson A, Lakey JH. Recombinant anthrax protective antigen: Observation of aggregation phenomena by TEM reveals specific effects of sterols. Micron 2017, 93, 1-8.
  • Clifton L, Ciesielski F, Skoda M, Paracini N, Holt S, Lakey J. The Effect of Lipopolysaccharide Core Oligosaccharide Size on the Electrostatic Binding of Antimicrobial Proteins to Models of the Gram Negative Bacterial Outer Membrane. Langmuir 2016, 32(14), 3485-3494.
  • Arunmanee W, Pathania M, Solovyova AS, Le Brun AP, Ridley H, Baslé A, van den Berg B, Lakey JH. Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis. Proceedings of the National Academy of Sciences of the USA 2016, 113(34), E5034-E5043.
  • Alfonsa H, Lakey JH, Lightowlers RN, Trevelyan AJ. Cl-out is a novel cooperative optogenetic tool for extruding chloride from neurons. Nature Communications 2016, 7, 13495.
  • LeBrun AP, Soliakov A, Shah DSH, Holt SA, McGill A, Lakey JH. Engineered self-assembling monolayers for label free detection of influenza nucleoprotein. Biomedical Microdevices 2015, 17(3), 49.
  • Clifton LA, Skoda MWA, Le Brun AP, Ciesielski F, Kuzmenko I, Holt SA, Lakey JH. Effect of Divalent Cation Removal on the Structure of Gram-Negative Bacterial Outer Membrane Models. Langmuir 2015, 31(1), 404-412.
  • Clifton LA, Holt SA, Hughes AV, Daulton EL, Arunmanee W, Heinrich F, Khalid S, Jefferies D, Charlton TR, Webster JRP, Kinane CJ, Lakey JH. An Accurate In Vitro Model of the E. coli Envelope. Angewandte Chemie International Edition 2015, 54(41), 11952-1195.
  • Johnson CL, Ridley H, Marchetti R, Silipo A, Griffin DC, Crawford L, Bonev B, Molinaro A, Lakey JH. The antibacterial toxin colicin N binds to the inner core of lipopolysaccharide and close to its translocator protein. Molecular Microbiology 2014, 92(3), 440-452.
  • Roque AI, Soliakov A, Birch MA, Philips SR, Shah DS, Lakey JH. Reversible Non-Stick Behaviour of a Bacterial Protein Polymer Provides a Tuneable Molecular Mimic for Cell and Tissue Engineering. Advanced Materials 2014, 26(17), 2704-2709.
  • Arunmanee W, Harris JR, Lakey JH. Outer Membrane Protein F Stabilised with Minimal Amphipol Forms Linear Arrays and LPS-Dependent 2D Crystals. Journal of Membrane Biology 2014, 247(9-10), 949-956.
  • Hughes AV, Holt SA, Daulton E, Soliakov A, Charlton TR, Roser SJ, Lakey JH. High coverage fluid-phase floating lipid bilayers supported by omega-thiolipid self-assembled monolayers. Journal of the Royal Society Interface 2014, 11(98).
  • Lu W, Zhang Y, McDonald DO, Jing HE, Carroll B, Robertson N, Zhang Q, Griffin H, Sanderson S, Lakey JH, Morgan NV, Reynard LN, Zheng L, Murdock HM, Turvey SE, Hackett SJ, Prestidge T, Hall JM, Cant AJ, Matthews HF, Koref MFS, Simon AK, Korolchuk VI, Lenardo MJ, Hambleton S, Su HC. Dual Proteolytic Pathways Govern Glycolysis and Immune Competence. Cell 2014, 159(7), 1578-1590.
  • Ridley H, Lakey JH. Antibacterial toxin colicin N and phage protein G3p compete with TolB for a binding site on TolA. Microbiology 2014, 161(3), 503-515.
  • Johnson CL, Ridley H, Pengelly RJ, Salleh MZ, Lakey JH. The unstructured domain of colicin N kills Escherichia coli. Molecular Microbiology 2013, 89(1), 84-95.
  • Le Brun AP, Clifton LA, Halbert CE, Lin B, Meron M, Holden PJ, Lakey JH, Holt SA. Structural Characterization of a Model Gram-Negative Bacterial Surface Using Lipopolysaccharides from Rough Strains of Escherichia coli. Biomacromolecules 2013, 14(6), 2014-2022.
  • Watkinson A, Soliakov A, Ganesan A, Hirst K, LeButt C, Fleetwood K, Fusco PC, Fuerst TR, Lakey JH. Increasing the Potency of an Alhydrogel-Formulated Anthrax Vaccine by Minimizing Antigen-Adjuvant Interactions. Clinical and Vaccine Immunology 2013, 20(11), 1659-1668.
  • Clifton LA, Skoda MWA, Daulton EL, Hughes AV, Le Brun AP, Lakey JH, Holt SA. Asymmetric phospholipid: lipopolysaccharide bilayers; a Gram-negative bacterial outer membrane mimic. Journal of the Royal Society Interface 2013, 10(89), 20130810.
  • Holt SA, Le Brun AP, Nelson ARJ, Lakey JH. In situ study of the impact of acidic and neutral deposition pH on alkane phosphate film formation and stability on TiO₂. RSC Advances 2013, 3(8), 2581-2589.
  • Marshall JL, Flores-Langarica A, Kingsley RA, Hitchcock JR, Ross EA, Lopez-Macias C, Lakey J, Martin LB, Toellner KM, MacLennan CA, MacLennan IC, Henderson IR, Dougan G, Cunningham AF. The Capsular Polysaccharide Vi from Salmonella Typhi Is a B1b Antigen. Journal of Immunology 2012, 189(12), 5527-5532.
  • Bavdek A, Kostanjsek R, Antonini V, Lakey JH, Serra MD, Gilbert RJC, Anderluh G. pH dependence of listeriolysin O aggregation and pore-forming ability. FEBS Journal 2012, 279(1), 126-141.
  • Clifton LA, Johnson CL, Solovyova AS, Callow P, Weiss KL, Ridley H, Le Brun AP, Kinane CJ, Webster JRP, Holt SA, Lakey JH. Low Resolution Structure and Dynamics of a Colicin-Receptor Complex Determined by Neutron Scattering. Journal of Biological Chemistry 2012, 287(1), 337-346.
  • Soliakov A, Kelly IF, Lakey JH, Watkinson A. Anthrax sub-unit vaccine: The structural consequences of binding rPA83 to Alhydrogel (R). European Journal of Pharmaceutics and Biopharmaceutics 2012, 80(1), 25-32.
  • Harris JR, Soliakov A, Lewis RJ, Depoix F, Watkinson A, Lakey JH. Alhydrogel® adjuvant, ultrasonic dispersion and protein binding: A TEM and analytical study. Micron 2012, 43(2-3), 192-200.
  • Le Brun AP, Holt SA, Shah DSH, Majkrzak CF, Lakey JH. The structural orientation of antibody layers bound to engineered biosensor surfaces. Biomaterials 2011, 32(12), 3303-3311.
  • Le Brun AP, Shah DSH, Athey D, Holt SA, Lakey JH. Self-Assembly of Protein Monolayers Engineered for Improved Monoclonal Immunoglobulin G Binding. International Journal of Molecular Sciences 2011, 12(8), 5157-5167.
  • Dickinson RE, Griffin H, Bigley V, Reynard LN, Hussain R, Haniffa M, Lakey JH, Rahman T, Wang XN, McGovern N, Pagan S, Cookson S, McDonald D, Chua I, Wallis J, Cant A, Wright M, Keavney B, Chinnery PF, Loughlin J, Hambleton S, Santibanez-Koref M, Collin M. Exome sequencing identifies GATA-2 mutation as the cause of dendritic cell, monocyte, B and NK lymphoid deficiency. Blood 2011, 118(10), 2656-2658.
  • Soliakov A, Harris JR, Watkinson A, Lakey JH. The structure of Yersinia pestis Caf1 polymer in free and adjuvant bound states. Vaccine 2010, 28(35), 5746-5754.
  • Chalton DA, Lakey JH. Simple Detection of Protein Soft Structure Changes. Analytical Chemistry 2010, 82(7), 3073-3076.
  • Cooke MJ, Zahir T, Phillips SR, Shah DSH, Athey D, Lakey JH, Shoichet MS, Przyborski SA. Neural differentiation regulated by biomimetic surfaces presenting motifs of extracellular matrix proteins. Journal of Biomedical Materials Research Part A 2010, 93A(3), 824-832.
  • Keegan N, Ridley H, Lakey J. Discovery of Biphasic Thermal Unfolding of OmpC with Implications for Surface Loop Stability. Biochemistry 2010, 49(45), 9715-9721.
  • Mitchell EA, Chaffey BT, McCaskie AW, Lakey JH, Birch MA. Controlled spatial and conformational display of immobilised bone morphogenetic protein-2 and osteopontin signalling motifs regulates osteoblast adhesion and differentiation in vitro. BMC Biology 2010, 8, -.
  • Bakrač B, Kladnik A, Maček P, McHaffie G, Werner A, Lakey JH, Anderluh G. A Toxin-based Probe Reveals Cytoplasmic Exposure of Golgi Sphingomyelin. Journal of Biological Chemistry 2010, 285(29), 22186-22195.
  • McDonald BM, Wydro MM, Lightowlers RN, Lakey JH. Probing the orientation of yeast VDAC1 in vivo. FEBS Letters 2009, 583(4), 739-742.
  • Hong Q, Rogero C, Lakey JH, Connolly BA, Houlton A, Horrocks BR. Immobilisation of proteins at silicon surfaces using undecenylaldehyde: demonstration of the retention of protein functionality and detection strategies. Analyst 2009, 134(3), 593-601.
  • Holt SA, Le Brun AP, Majkrzak CF, McGillivray DJ, Heinrich F, Losche M, Lakey JH. An ion-channel-containing model membrane: structural determination by magnetic contrast neutron reflectometry. Soft Matter 2009, 5(13), 2576-2586.
  • Hecht O, Ridley H, Lakey JH, Moore GR. A Common Interaction for the Entry of Colicin N and Filamentous Phage into Escherichia coli. Journal of Molecular Biology 2009, 388(4), 880-893.
  • Rogero C, Chaffey BT, Mateo-Martí E, Sobrado JM, Horrocks BR, Houlton A, Lakey JH, Briones C, Martín-Gago JA. Silicon Surface Nanostructuring for Covalent Immobilization of Biomolecules. Journal of Physical Chemistry C 2008, 112(25), 9308-9314.
  • Hecht O, Ridley H, Boetzel R, Lewin A, Cull N, Chalton DA, Lakey JH, Moore GR. Self-recognition by an intrinsically disordered protein. FEBS Letters 2008, 582(17), 2673-2677.
  • Le Brun AP, Holt SA, Shah DS, Majkrzak CF, Lakey JH. Monitoring the assembly of antibody-binding membrane protein arrays using polarised neutron reflection. European Biophysics Journal 2008, 37(5), 639-645.
  • Bakrač B, Gutiérrez-Aguirre I, Podlesek Z, Sonnen AFP, Gilbert RJC, Maček P, Lakey JH, Anderluh G. Molecular determinants of sphingomyelin specificity of a eukaryotic pore-forming toxin. Journal of Biological Chemistry 2008, 283(27), 18665-18677.
  • Nedelkina S, Gokce I, Ridley H, Weckerle C, Magnin T, Vallette F, Pattus F, Lakey JH, Bechinger B. High-yield expression and purification of soluble forms of the anti-apoptotic Bcl-x_L and Bcl-2 as TolAIII-fusion proteins. Protein Expression and Purification 2008, 60(2), 214-220.
  • Cooke MJ, Phillips SR, Shah DSH, Athey D, Lakey JH, Przyborski SA. Enhanced cell attachment using a novel cell culture surface presenting functional domains from extracellular matrix proteins. Cytotechnology 2008, 56(2), 71-79.
  • Dumon C, Varvak A, Wall MA, Flint JE, Lewis RJ, Lakey JH, Morland C, Luginbuhl P, Healey S, Todaro T, DeSantis G, Sun M, Parra-Gessert L, Tan XQ, Weiner DP, Gilbert HJ. Engineering hyperthermostability into a GH₁₁ xylanase is mediated by subtle changes to protein structure. Journal of Biological Chemistry 2008, 283(33), 22557-22564.
  • Baboolal TG, Conroy MJ, Gill KL, Ridley H, Visudtiphole V, Bullough PA, Lakey JH. Colicin N binds to the periphery of its receptor and translocator, outer membrane protein F. Structure 2008, 16(3), 371-379.
  • Chaffey BT, Mitchell E, Birch MA, Lakey JH. A generic expression system to produce proteins that co-assemble with alkane thiol SAM. International Journal of Nanomedicine 2008, 3(3), 287-293.
  • Chalton DA, Kelly IF, McGregor A, Ridley H, Watkinson A, Miller J, Lakey JH. Unfolding transitions of Bacillus anthracis protective antigen. Archives of Biochemistry and Biophysics 2007, 465(1), 1-10.
  • Bavdek A, Gekara NO, Priselac D, Aguirre IG, Darji A, Chakraborty T, Macek P, Lakey JH, Weisse S, Anderluh G. Sterol and pH interdependence in the binding, oligomerization, and pore formation of listeriolysin O. Biochemistry 2007, 46(14), 4425-4437.
  • Aisenbrey C, Sudheendra US, Ridley H, Bertani P, Marquette A, Nedelkina S, Lakey JH, Bechinger B. Helix orientations in membrane-associated Bcl-XL determined by 15N-solid-state NMR spectroscopy. European Biophysics Journal 2007, 37(1), 71-80.
  • Kirmizidis G, Lakey JH, Birch MA. Differential vascular endothelial growth factor receptor expression during osteogenesis. Journal of Bone and Mineral Research 2007, 22(7), 1128-1128.
  • Leontiou C, Watters GP, Gilroy KL, Heslop P, Cowell IG, Craig K, Lightowlers RN, Lakey JH, Austin CA. Differential selection of acridine resistance mutations in human DNA topoisomerase II beta is dependent on the acridine structure. Molecular Pharmacology 2007, 71(4), 1006-1014.
  • Weir KS, Tharia H, Hitchcock T, Lakey JH. Critical factors in the in vitro refolding pathway of IFN-alpha 2b. Biophysical Journal 2007, 399A-399A.
  • Xie HF, Flint JE, Vardakou M, Lakey JH, Lewis RJ, Gilbert HJ, Dumon C. Probing the Structural Basis for the Difference in Thermostability Displayed by Family 10 Xylanases. Journal of Molecular Biology 2006, 360(1), 157-167.
  • Keresztessy Z, Csosz E, Harsfalvi J, Csomos K, Gray J, Lightowlers RN, Lakey JH, Balajthy Z, Fesus L. Phage display selection of efficient glutamine-donor substrate peptides for transglutaminase 2. Protein Science 2006, 15(11), 2466-2480.
  • Leontiou C, Lakey JH, Lightowlers R, Turnbull RM, Austin CA. Mutation P732L in human DNA topoisomerase II abolishes DNA cleavage in the presence of calcium and confers drug resistance. Molecular Pharmacology 2006, 69(1), 130-139.
  • Gutierrez-Aguirre I, Trontelj P, Macek P, Lakey JH, Anderluh G. Membrane binding of zebrafish actinoporin-like protein: AF domains, a novel superfamily of cell membrane binding domains. Biochemical Journal 2006, 398(3), 381-392.
  • Gilroy KL, Leontiou C, Padget K, Lakey JH, Austin CA. mAMSA resistant human topoisomerase IIβ mutation G465D has reduced ATP hydrolysis activity. Nucleic Acids Research 2006, 34(5), 1597-1607.
  • Race PR, Lakey JH, Banfield MJ. Insertion of the enteropathogenic Escherichia coli Tir virulence protein into membranes in vitro. Journal of Biological Chemistry 2006, 281(12), 7842-7849.
  • Chalton DA, Musson JA, Flick-Smith H, Walker N, McGregor A, Lamb HK, Williamson ED, Miller J, Robinson JH, Lakey JH. Immunogenicity of a Yersinia pestis vaccine antigen monomerized by circular permutation. Infection and Immunity 2006, 74(12), 6624-6631.
  • von Delwig A, Wilson AJ, Altmann DM, Holmdahl R, Harding CV, Mckie N, Isaacs JD, Lakey JH, Robinson JH. Febrile temperatures enhance antigen processing of type II collagen for presentation to CD4 T cells. Arthritis & Rheumatism 2006, 54(9), S639-S639.
  • Visudtiphole V, Chalton DA, Hong Q, Lakey JH. Determining OMP topology by computation, surface plasmon resonance and cysteine labelling: The test case of OMPG. Biochemical and Biophysical Research Communications 2006, 351(1), 113-117.
  • Cisneros DA, Muller DJ, Daud SM, Lakey JH. An approach to prepare membrane proteins for single-molecule imaging. Angewandte Chemie - International Edition 2006, 45(20), 3252-3256.
  • Gokce I, Woody RW, Anderluh G, Lakey JH. Single peptide bonds exhibit poly(Pro)II ("random coil") circular dichroism spectra. Journal of the American Chemical Society 2005, 127(27), 9700-9701.
  • Visudtiphole V, Thomas MB, Chalton DA, Lakey JH. Refolding of Escherichia coli outer membrane protein F in detergent creates LPS-free trimers and asymmetric dimers. Biochemical Journal 2005, 392(2), 375-381.
  • Anderluh G, Besenicar M, Kladnik A, Lakey JH, Macek P. Properties of nonfused liposomes immobilized on an L1 Biacore chip and their permeabilization by a eukaryotic pore-forming toxin. Analytical Biochemistry 2005, 344(1), 43-52.
  • Holt SA, Lakey JH, Daud SM, Keegan N. Neutron reflectometry of membrane protein assemblies at the solid/liquid interface. Australian Journal of Chemistry 2005, 58(9), 674-677.
  • Keegan N, Wright NG, Lakey JH. Circular dichroism spectroscopy of folding in a protein monolayer. Angewandte Chemie - International Edition 2005, 44(30), 4801-4804.
  • Andrews SR, Taylor EJ, Pell G, Vincent F, Ducros VM-A, Davies GJ, Lakey JH, Gilbert HJ. The use of forced protein evolution to investigate and improve stability of family 10 xylanases: The production of Ca^₂₊independent stable xylanases. Journal of Biological Chemistry 2004, 279(52), 54369-54379.
  • Lax AJ, Pullinger GD, Baldwin MR, Harmey D, Grigoriadis AE, Lakey JH. The Pasteurella multocida toxin interacts, with signalling pathways to perturb cell growth and differentiation. International Journal of Medical Microbiology 2004, 293(7-8), 505-512.
  • Kristan K, Podlesek Z, Hojnik V, Gutierrez-Aguirre I, Guncar G, Turk D, Gonzalez-Manas JM, Lakey JH, Macek P, Anderluh G. Pore formation by equinatoxin, a eukaryotic pore-forming toxin, requires a flexible N-terminal region and a stable β-sandwich. Journal of Biological Chemistry 2004, 279(45), 46509-46517.
  • Leontiou C, Lakey JH, Austin CA. Mutation E522K in human DNA topoisomerase IIβ confers resistance to methyl N-(4′-(9-acridinylamino)-phenyl)carbamate hydrochloride and methyl N-(4′-(9-acridinylamino)-3-methoxy-phenyl) methane sulfonamide but hypersensitivity to etoposide. Molecular Pharmacology 2004, 66(3), 430-439.
  • Baldwin MR, Lakey JH, Lax AJ. Identification and characterization of the Pasteurella multocida toxin translocation domain. Molecular Microbiology 2004, 54(1), 239-250.
  • Anderluh G, Gokce I, Lakey JH. A Natively Unfolded Toxin Domain Uses Its Receptor as a Folding Template. Journal of Biological Chemistry 2004, 279(21), 22002-22009.
  • Nagy T, Nurizzo D, Davies G, Biely P, Lakey JH, Bolam DN, Gilbert HJ. The α-Glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants. Journal of Biological Chemistry 2003, 278(22), 20286-20292.
  • Gokce I, Lakey JH. Production of an E. coli toxin protein; Colicin A in E. coli using an inducible system. Turkish Journal of Chemistry 2003, 27(3), 323-331.
  • Leontiou C, Lightowlers R, Lakey JH, Austin CA. Kinetic analysis of human topoisomerase IIα and β DNA binding by surface plasmon resonance. FEBS Letters 2003, 554(1-2), 206-210.
  • Anderluh G, Gokce I, Lakey JH. Expression of proteins using the third domain of the Escherichia coli periplasmic-protein TolA as a fusion partner. Protein Expression and Purification 2003, 28(1), 173-181.
  • Anderluh G, Hong Q, Boetzel R, MacDonald C, Moore GR, Virden R, Lakey JH. Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA. Journal of Biological Chemistry 2003, 278(24), 21860-21868.
  • Malovrh P, Viero G, Dalla Serra M, Podlesek Z, Lakey JH, Macek P, Menestrina G, Anderluh G. A novel mechanism of pore formation: Membrane penetration by the N-terminal amphipathic region of equinatoxin. Journal of Biological Chemistry 2003, 278(25), 22678-22685.
  • Hong Q, Gutierrez-Aguirre I, Barli A, Malovrh P, Kristan K, Podlesek Z, Maek P, Turk D, Gonzalez-Manas JM, Lakey JH, Anderluh G. Two-step membrane binding by equinatoxin II, a pore-forming toxin from the sea anemone, involves an exposed aromatic cluster and a flexible helix. Journal of Biological Chemistry 2002, 277(44), 41916-41924.
  • Fridd SL, Lakey JH. Surface aspartate residues are essential for the stability of colicin A P-domain: A mechanism for the formation of an acidic molten-globule. Biochemistry 2002, 41(5), 1579-1586.
  • Terrettaz S, Ulrich W-P, Vogel H, Hong Q, Dover LG, Lakey JH. Stable self-assembly of a protein engineering scaffold on gold surfaces. Protein Science 2002, 11(8), 1917-1925.
  • Anderluh G, Gokce I, Lakey JH. Expression of fusion proteins using the third domain of the periplasmic protein TolA from E. coli as a fusion partner. Protein Expression and Purification 2002, 28, 178-183.
  • West, K. L., Turnbull, R. M., Willmore, E., Lakey, J. H., Austin, C. A. Characterisation of the DNA-dependent ATPase activity of human DNA topoisomerase IIß: mutation of Ser165 in the ATPase domain reduces the ATPase activity and abolishes the in vivo complementation ability. Nucleic Acids Research 2002, 30(24), 5416-5424.
  • Xie H, Bolam DN, Nagy T, Szabó L, Cooper A, Simpson P, Lakey J, Williamson M, Gilbert H. Role of hydrogen bonding in the interaction between a xylan binding module and xylan. Biochemistry 2001, 40(19), 5700-5707.
  • Lakey JH, Slatin SL. Pore-forming colicins and their relatives. Current topics in Microbiology and Immunology 2001, 257, 131-161.
  • Snowdon K, McNeil C, Lakey J. Nanotechnology for MEMS Components. MST News 2001, 3(1), 9-10.
  • Paquet JY, Diaz MA, Genevrois S, Grayon M, Verger JM, de Bolle X, Lakey JH, Letesson JJ, Cloeckaert A. Molecular, antigenic, and functional analyses of Omp2b porin size variants of Brucella spp. Journal of Bacteriology 2001, 183(16), 4839-4847.
  • Lakey JH, Reddy BV, Murray-Rust J. Macromolecular assemblages. Theory and simulation. Current Opinion in Structural Biology 2001, 11(2), 139-140.
  • Cooper A, Johnson CM, Lakey JH, Nollmann M. Heat does not come in different colours: Entropy-enthalpy compensation, free energy windows, quantum confinement, pressure perturbation calorimetry, solvation and the multiple causes of heat capacity effects in biomolecular interactions. Biophysical Chemistry 2001, 93(2-3), 215-230.
  • Hong Q, Terrettaz S, Ulrich WP, Vogel H, Lakey JH. Assembly of pore proteins on gold electrodes. Biochemical Society Transactions 2001, 29(4), 578-582.
  • Bourne PE, Murray-Rust J, Lakey JH. Web alert: Engineering and design: Membrane proteins: Lipids. Current Opinion in Structural Biology 2000, 10(4), 397-398.
  • Kremer L, Douglas JD, Baulard AR, Morehouse C, Guy MR, Alland D, Dover LG, Lakey JH, Jacobs Jr WR, Brennan PJ, Minnikin DE, Besra GS. Thiolactomycin and Related Analogues as Novel Anti-mycobacterial Agents Targeting KasA and KasB Condensing Enzymes in Mycobacterium tuberculosis. Journal of Biological Chemistry 2000, 275(22), 16857-16864.
  • Gokce I, Raggett EM, Hong Q, Virden R, Cooper A, Lakey JH. The TolA-recognition site of colicin N. ITC, SPR and stopped-flow fluorescence define a crucial 27-residue segment. Journal of Molecular Biology 2000, 304(4), 621-632.
  • Stephenson K, Jensen CL, Jorgensen ST, Lakey JH, Harwood CR. The influence of secretory-protein charge on late stages of secretion from the Gram-positive bacterium Bacillus subtilis. Biochemical Journal 2000, 350(1), 31-39.
  • Andrews SR, Charnock SJ, Lakey JH, Davies GJ, Claeyssens M, Nerinckx W, Underwood M, Sinnott ML, Warren RAJ, Gilbert HJ. Substrate specificity in glycoside hydrolase family 10: Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of pseudomonas cellulosa xylanase 10A. Journal of Biological Chemistry 2000, 275(30), 23027-23033.
  • Dover LG, Evans LJA, Fridd SL, Bainbridge G, Raggett EM, Lakey JH. Colicin pore-forming domains bind to Escherichia coli trimeric porins. Biochemistry 2000, 39(29), 8632-8637.
  • Lakey JH, Tate J, Murray-Rust J. Carbohydrates and glycoconjugates. Biophysical methods. Current Opinions in Structural Biology 2000, 10(5), 505-506.
  • Yang SH, Shore P, Willingham N, Lakey JH, Sharrocks AD. The mechanism of phosphorylation-inducible activation of the ETS-domain transcription factor Elk-1. EMBO Journal 1999, 18(20), 5666-5674.
  • Monchois V, Lakey JH, Russell RRB. Secondary structure of Streptococcus downei GTF-I glucansucrase. FEMS Microbiology Letters 1999, 177(2), 243-248.
  • Stora T, Lakey JH, Vogel H. Ion-channel gating in transmembrane receptor proteins: Functional activity in tethered lipid membranes. Angewandte Chemie: International Edition 1999, 38(3), 389-392.
  • G. Bainbridge, H. Mobasheri, G. A. Armstrong, E. J. A. Lea and J. H. Lakey. Voltage-gating of Escherichia coli porin: A cystine-scanning mutagenesis study of loop 3. Journal of Molecular Biology 1998, 275, 171-176.
  • G Bainbridge,H Mobasheri,G.A. Armstrong,E.J.A Lea,J.H. Lakey. Voltage gating of Escherichia coli porin; a cystine scanning mutagenesis study of loop 3. Journal Of Molecular Biology 1998, 275, 171-176.
  • G. Bainbridge, I. Gokce and J. H. Lakey. Voltage gating is a fundamental feature of porin and toxin beta-barrel membrane channels. Febs Letters 1998, 431, 305-308.
  • G Bainbridge,I Gokce,J.H. Lakey. Voltage gating is a fundamental feature of Porin and toxin b-barrel membrane channels. FEBS Letters 1998, 431, 305-308.
  • J.H Lakey,E.M. Raggett. Measuring protein-protein interactions. Curr. Opin. Struc. Biol 1998, 8(1), 119-123.
  • J. H. Lakey and E. M. Raggett. Measuring protein-protein interactions. Current Opinion in Structural Biology 1998, 8, 119-123.
  • J. Miller, E. D. Williamson, J. H. Lakey, M. J. Pearce, S. M. Jones and R. W. Titball. Macromolecular organisation of recombinant Yersinia pestis F1 antigen and the effect of structure on immunogenicity. Fems Immunology and Medical Microbiology 1998, 21, 213-221.
  • J. Miller,E. D. Willamson,J. H. Lakey,R. W. Titball. Macromolecular organisation of recombinant F1 antigen and the effect of structure on immunogenicity. FEMS Immunol. and Med. Microbiol 1998, 21(3), 213-222.
  • Y Ling,A.G. West,E.C. Roberts,J.H. Lakey,A.D. Sharrocks. Interaction of transcription factors with SRF:identification of the Elk-1 binding surface. Journal of Biological Chemistry 1998, 273(17), 10506-10514.
  • Y. Ling, A. G. West, E. C. Roberts, J. H. Lakey and A. D. Sharrocks. Interaction of transcription factors with serum response factor - Identification of the Elk-1 binding surface. Journal of Biological Chemistry 1998, 273, 10506-10514.
  • G Bainbridge,G.A. Armstrong,L.G. Dover,K.F. Whelan,J.H. Lakey. Displacement of OmpF loop 3 is not required for the membrane translocation of colicins N and A in vivo. FEBS Letters 1998, 432, 117-122.
  • G. Bainbridge, G. A. Armstrong, L. G. Dover, K. F. Whelan and J. H. Lakey. Displacement of OmpF loop 3 is not required for the membrane translocation of colicins N and A in vivo. Febs Letters 1998, 432, 117-122.
  • E.M. Raggett,G Bainbridge,L.J.E. Evans,A Cooper,J.H. Lakey. Discovery of critical Tol A-binding residues in the bactericidal toxin colicin N; a biophysical approach. Molecular Microbiology 1998, 28(6), 1335-1344.
  • E. M. Raggett, G. Bainbridge, L. J. A. Evans, A. Cooper and J. H. Lakey. Discovery of critical tol A-binding residues in the bactericidal toxin colicin N: a biophysical approach. Molecular Microbiology 1998, 28, 1335-1343.
  • I.R. Vetter,M.W. Parker,A.D. Tucker,J.H. Lakey,F. Pattus,D Tsernoglou. Crystal structure of a colicin N fragment suggests a model for toxicity. Structure 1998, 6, 863-874.
  • I. R. Vetter, M. W. Parker, A. D. Tucker, J. H. Lakey, F. Pattus and D. Tsernoglou. Crystal structure of a colicin N fragment suggests a model for toxicity. Structure with Folding & Design 1998, 6, 863-874.
  • A.J Bird,J.S. Turner-Cavet,J.H. Lakey,N.J. Robinson. A carboxy-terminal Cys2/His2 type zinc-finger motif in DNA primase influences DNA content in Synechococcus PCC 7942. Journal of Biological Chemistry 1998, 273(33), 21246-21252.
  • Bird AJ, Turner-Cavet JS, Lakey JH, Robinson NJ. A carboxyl-terminal Cys2/His2-type zinc-finger motif in DNA primase influences DNA content in Synechococcus PCC 7942. Journal of Biological Chemistry 1998, 273(33), 21246-21252.
  • I. Gokce,G. Bainbridge,J. H. Lakey. Stabilising and destabilising modifications of cysteines in the E-coli outer membrane porin protein OmpC. Febs Letters 1997, 411(2-3), 201-205.
  • I. Gokce, G. Bainbridge and J. H. Lakey. Stabilising and destabilising modifications of cysteines in the E-coli outer membrane porin protein OmpC. Febs Letters 1997, 411, 201-205.
  • J. Hughes,J. H. Lakey,M. A. Hughes. Production and characterization of a plant alpha-hydroxynitrile lyase in Escherichia coli. Biotechnology And Bioengineering 1997, 53(3), 332-338.
  • Y. Ling, J. H. Lakey, C. E. Roberts and A. D. Sharrocks. Molecular characterization of the B-box protein-protein interaction motif of the ETS-domain transcription factor Elk-1. Embo Journal 1997, 16, 2431-2440.
  • Y Ling,J.H. Lakey,C.E. Roberts,A.D Sharrocks. Molecular characterisation of the B-box protein-protein interaction motif of the ETS-domain transcription factor Elk1. EMBO Journal 1997, 16(9), 2431-2440.
  • J Miller,E.D. Willamson,J.H. Lakey,R.W. Titball. Macromolecular organisation of recombinant F1 antigen. Protein Science 1997, 6(1), A113.
  • Miller J, Willamson ED, Lakey JH, Titball RW. Macromolecular organisation of recombinant F1 antigen. PROTEIN SCIENCE, 1997, Vol.6, A113<br>(Article) 1997.
  • S. J. Charnock,J. H. Lakey,R. Virden,N. Hughes,M. L. Sinnott,G. P. Hazlewood,R. Pickersgill,H. J. Gilbert. Key residues in subsite F play a critical role in the activity of Pseudomonas fluorescens subspecies cellulosa xylanase A against xylooligosaccharides but not against highly polymeric substrates such as xylan. Journal Of Biological Chemistry 1997, 272(5), 2942-2951.
  • K. L. Braithwaite,T. Barna,T. D. Spurway,S. J. Charnock,G. W. Black,N. Hughes,J. H. Lakey,R. Virden,G. P. Hazlewood,B. Henrissat,H. J. Gilbert. Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues. Biochemistry 1997, 36(49), 15489-15500.
  • G Bainbridge,G.A. Armstrong,H Mobasheri,E.J.A. Lea,Lakey J.H. Channel and Biochemical Properties of Bacterial Porin Mutants. Biophys. J 1997, 72(2), A138.
  • H. Mobasheri, T. A. Ficht, H. Marquis, E. J. A. Lea and J. H. Lakey. Brucella Omp2a and Omp2b porins: single channel measurements and topology prediction. Fems Microbiology Letters 1997, 155, 23-30.
  • Mobasheri H, Ficht TA, Marquis H, Lea EJA, Lakey JH. Brucella Omp2a and Omp2b porins: single channel measurements and topology prediction. FEMS MICROBIOLOGY LETTERS, 1997, Vol.155, No.1, pp.23-30<br>Issn: 0378-1097<br>(Article) 1997.
  • H. Mobasheri,T.A. Ficht,H. Marquis,E.J.A. Lea,J.H. Lakey. Brucella omp2a and 2b porins; single channel measurements and topology prediction. FEMS Microbiology Letters 1997, 155, 23-30.
  • E.M. Raggett,G. Bainbridge,L.J.A Evans,A Cooper,J.H. Lakey. Binding constants for the TolA/Colicin N protein-protein recognition system. Protein Science 1997, 6, 63.
  • H. Mobasheri,E.J.A Lea,T.A. Ficht,H. Marquis,J.H. Lakey. Bacterial porin channels and voltage dependence. Experiments with two Brucella porins, one missing a 36 amino acid segment. Biophys. J 1997, 72(2), A138.
  • H. Mobasheri, E. J. A. Lea, T. Ficht, H. Marquis and J. H. Lakey. Bacterial porin channels and voltage dependence experiments with two Brucella abortus porins, one missing a 36 amino acid segment. Biophysical Journal 1997, 72, TUPM4-TUPM4.
  • H. Mobasheri,Eja Lea,T. Ficht,H. Marquis,J. H. Lakey. Bacterial porin channels and voltage dependence experiments with two Brucella abortus porins, one missing a 36 amino acid segment. Biophysical Journal 1997, 72(2), TUPM4-TUPM4.
  • L.J.A Evans,S Labeit,A Cooper,L.H. Bond,J.H. Lakey. The central domain of colicin N possesses the receptor recognition site but not the binding affinity of the whole toxin. Biochemistry 1996, 35(48), 15143-15148.
  • L. J. A. Evans, S. Labeit, A. Cooper, L. H. Bond and J. H. Lakey. The central domain of colicin N possesses the receptor recognition site but not the binding affinity of the whole toxin. Biochemistry 1996, 35, 15143-15148.
  • D.T. Flaxman,V Steinthorsdottir,M.A. Kehoe,J.H. Lakey. Structural studies on Clostridium perfringens b-toxin: Theoretical and practical approaches. Medical Microbiology and Immunology 1996, 185(2), 113.
  • Bolam DN, Hughes N, Virden R, Lakey JH, Hazlewood G, Henrissat B, Braithwaite K, Gilbert HJ. Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues. Biochemistry 1996, 35(50), 16195-16204.
  • P Jasperse,J.H. Lakey. Investigation of the Structure of the IsK channel. Progress in Biophysics and Molecular Biology 1996, 65(s1), 313.
  • L.J.A Evans,A Cooper,J.H. Lakey. Direct measurement of the association of a protein with a family of membrane receptors. Journal Of Molecular Biology 1996, 255(4), 559-563.
  • L. J. A. Evans, A. Cooper and J. H. Lakey. Direct measurement of the association of a protein with a family of membrane receptors. Journal of Molecular Biology 1996, 255, 559-563.
  • L.J.A Evans,M.L. Goble,K. Hales,J.H. Lakey. Different sensitivities to acid denaturation within a family of proteins; Implications for acid unfolding and membrane translocation. Biochemistry 1996, 35(40), 13180-13185.
  • L. J. A. Evans, M. L. Goble, K. A. Hales and J. H. Lakey. Different sensitivities to acid denaturation within a family of proteins: Implications for acid unfolding and membrane translocation. Biochemistry 1996, 35, 13180-13185.
  • H. K. Lamb, J. D. Moore, J. H. Lakey, L. J. Levett, K. A. Wheeler, H. Lago, J. R. Coggins and A. R. Hawkins. Comparative analysis of the QUTR transcription repressor protein and the three C-terminal domains of the pentafunctional AROM enzyme. Biochemical Journal 1996, 313, 941-950.
  • H.K. Lamb,J.D. Moore,J.H. Lakey,L Levett,K.A. Wheeler,H. Lago,J.R. Coggins,A.R. Hawkins. Comparative analysis of the QUTR transcription repressor protein and the 3 C-terminal domains of the pentafunctional AROM enzyme. Biochemical Journal 1996, 313, 941 - 950.
  • L.J.A Evans,E. Raggett,A Cooper,S Labeit,J.H. Lakey. Colicin N: Receptor Binding and Translocation. Medical Microbology and Immunology 1996, 185(2), 112.
  • L.J.A Evans,J.H. Lakey. Investigation of the receptor binding properties of colicin N. Protein Science 1995, 4(S1), A338.
  • M. P. Hornshaw,J. R. Mcdermott,J. M. Candy,J. H. Lakey. Copper-binding to the n-terminal tandem repeat region of mammalian and avian prion protein - structural studies using synthetic peptides. Biochemical And Biophysical Research Communications 1995, 214(3), 993-999.
  • P Shore,L Bisset,J.H. Lakey,J.P Waltho,R Virden,A.D Sharrocks. Characterization of the Elk-1 ETS DNA-binding Domain. Journal of Biological Chemistry 1995, 270(11), 5805-5811.
  • P. Shore,L. Bisset,J. Lakey,J. P. Waltho,R. Virden,A. D. Sharrocks. Characterization of the Elk-1 Ets DNA-Binding Domain. Journal of Biological Chemistry 1995, 270(11), 5805-5811.
  • J. H. Lakey,M.W. Parker,J. M. Gonzalez Manas,D Duche,G Vriend,D Baty,F. Pattus. The role of electrostatic charge in the membrane insertion of colicin A: calculation and mutation. European Journal Of Biochemistry 1994, 220, 155-163.
  • Lakey JH, van der Goot FG, Pattus F. All in the family: the toxic activity of pore-forming colicins. TOXICOLOGY, 1994, Vol.87, No.1-3, pp.85-108<br>(Review) 1994.
  • J.H. Lakey,F.G. van der Goot,F. Pattus. All in the family: the toxic activity of colicins. Toxicology 1994, 87, 85-108.
  • A. Muga,J. M. Gonzaléz-Mañas,J. H. Lakey,F. Pattus,W. K. Surewicz. pH-dependent stability and membrane interaction of the pore-forming domain of colicin-A. Journal Of Biological Chemistry 1993, 268(3), 1553-1557.
  • A. Muga,J. M. Gonzalez-Manas,J. H. Lakey,F. Pattus,W. K. Surewicz. pH dependent stability and membrane interaction of the pore forming domain of colicin-a. Biophysical Journal 1993, 288.
  • J.M. González-Mañas,J.H. Lakey,F. Pattus. Interaction of the colicin-A pore-forming domain with negatively charged phospholipids. European Journal Of Biochemistry 1993, 211(3), 625-633.
  • J. H. Lakey,D. Duché,J-M. González-Mañas,D. Baty,F. Pattus. Fluorescence energy transfer distance measurements: the hydrophobic helical hairpin of Colicin A in the membrane bound state. J Mol Biol 1993, 230(3), 1055-1067.
  • Eja Lea,N. D. Bishop,J. H. Lakey. Escherichia-coli porin channels, fast transitions and voltage dependence. Biophysical Journal 1993, 64(2), 94.
  • F. Lesage,B. Attali,J. Lakey,E. Honore,G. Romey,E. Faurobert,M. Lazdunski,J. Barhanin. Are Xenopus oocytes unique in displaying functional Isk Cannel heterologous expression?. Receptors and Channels 1993, 1, 143-152.
  • J.H. Lakey. 2nd international workshop on pore-forming toxins september 29 october 2, 1993 (mainz, germany) - abstracts. Medical Microbiology And Immunology 1993, 182(4), 177-221.
  • F.G. van der Goot,J.H. Lakey,F. Pattus. The molten globule intermediate for protein insertion or translocation through membranes. Trends in Cell Biology 1992, 2(11), 343-348.
  • J. H. Lakey,J.M. González-Mañas,F.G. van der Goot,F. Pattus. The membrane insertion of colicins. Febs Lett 1992, 307(1), 26-9.
  • F. G. Vandergoot,J. Lakey,F. Pattus,C. M. Kay,O. Sorokine,A. Vandorsselaer,J. T. Buckley. Spectroscopic Study of the Activation and Oligomerization of the Channel-Forming Toxin Aerolysin - Identification of the Site of Proteolytic Activation. Biochemistry 1992, 31(36), 8566-8570.
  • F. G. van der Goot,J. Lakey,F. Pattus,C. M. Kay,O. Sorokine,A. Vandorsselaer,J. T. buckley. Spectroscopic study of the activation and oligomerization of the channel-forming toxin aerolysin - identification of the site of proteolytic activation. Biochemistry 1992, 31(36), 8566-8570.
  • E.J.A. Lea,J. H. Lakey. Escherichia coli ompC porin channels exhibit fast transitions. Faseb Journal 1992, 6.
  • J.M. González-Mañas,J. H. Lakey,F. Pattus. Brominated phospholipids as a tool for monitoring the membrane insertion of colicin A. Biochemistry 1992, 31(32), 7294-300.
  • D. Jeanteur,J. H. Lakey,F. Pattus. The bacterial porin superfamily: sequence alignment and structure prediction. Molecular Microbiology 1991, 5(9), 2153-2164.
  • J.H. Lakey,E.J.A. Lea,F. Pattus. ompC Mutants Which Allow Growth on Maltodextrins Show Increased Channel Size and Greater Voltage Sensitivity. FEBS Letters 1991, 278(1), 31-34.
  • J. H. Lakey,D. Massotte,F. Heitz,J. L. Dasseux,J. F. Faucon,M. W. Parker,F. Pattus. Membrane insertion of the pore-forming domain of colicin A. A spectroscopic study. Eur J Biochem 1991, 196(3), 599-607.
  • J. H. Lakey,D. Baty,F. Pattus. Fluorescence energy transfer distance measurements using site-directed single cysteine mutants. The membrane insertion of colicin A. J Mol Biol 1991, 218(3), 639-53.
  • F.G. van der Goot,J.M. González-Mañas,J. H. Lakey,F. Pattus. A 'molten-globule' membrane-insertion intermediate of the pore-forming domain of colicin A. Nature 1991, 354(6352), 408-10.
  • F. Pattus,D. Massotte,H. U. Wilmsen,J. Lakey,D. Tsernoglou,A. Tucker,M. W. Parker. Colicins: prokaryotic killer-pores. Experientia 1990, 46(2), 180-92.
  • F. Pattus,D. Massotte,H. U. Wilmsen,J. Lakey,D. Tsernoglou,A. Tucker,M. W. Parker. Colicins - Prokaryotic Killer-Pores. Experientia 1990, 46(2), 180-192.
  • D. Baty,J. Lakey,F. Pattus,C. Lazdunski. A 136-Amino-Acid-Residue Cooh-Terminal Fragment of Colicin-a Is Endowed with Ionophoric Activity. European Journal of Biochemistry 1990, 189(2), 409-413.
  • D. Baty,J. Lakey,F. Pattus,C. Lazdunski. A 136-amino-acid-residue COOH-terminal fragment of colicin A is endowed with ionophoric activity. Eur J Biochem 1990, 189(2), 409-13.
  • J. H. Lakey,F. Pattus. The voltage-dependent activity of Escherichia coli porins in different planar bilayer reconstitutions. European Journal of Biochemistry 1989, 186, 303-308.
  • J. H. Lakey,R. Maget-Dana,M. Ptak. The lipopeptide antibiotic A21978C has a specific interaction with DMPC only in the presence of calcium ions. Biochim Biophys Acta 1989, 985(1), 60-6.
  • J. Lakey,F. Pattus. A Surprising Omission. Trends in Biochemical Sciences 1989, 14(1), 14-14.
  • J. H. Lakey,M. Ptak. Fluorescence indicates a calcium-dependent interaction between the lipopeptide antibiotic LY146032 and phospholipid membranes. Biochemistry 1988, 27(13), 4639-45.
  • J. H. Lakey,R. Maget-Dana,M. Ptak. Conformational change on calcium-binding by the lipopeptide antibiotic amphomycin - a CD and monolayer study. Biochemical And Biophysical Research Communications 1988, 150(1), 384-390.
  • F. Pattus,J. Lakey,B. Dargent. Bacterial proteins which form membrane pores - a biophysical approach. Microbiological Sciences 1988, 5(7), 207-210.
  • R. Maget-Dana,J. H. Lakey,M. Ptak. A comparative monomolecular film study of antibiotic A21978c homologs of various lipid chain-length. Biochimica Et Biophysica Acta 1988, 962(2), 201-207.
  • J. H. Lakey. Voltage gating in porin channels. FEBS Letters 1987, 211(1), 1-4.
  • J. H. Lakey,Eja Lea. The role of acyl chain character and other determinants on the bilayer activity of a21978c an acidic lipopeptide antibiotic. Biochimica Et Biophysica Acta 1986, 859(2), 219-226.
  • J. H. Lakey,J. P. Watts,E.J.A Lea. Characterization of channels induced in planar bilayer-membranes by detergent solubilized Escherichia coli porins. Biochimica Et Biophysica Acta 1985, 817(2), 208-216.
  • J. H. Lakey. Calmodulin and eukaryote evolution. Nature 1985, 315(6022), 720.
  • J. H. Lakey,Eja Lea,Bam Rudd,H. M. Wright,D. A. Hopwood. A new channel-forming antibiotic from streptomyces-coelicolor a3(2) which requires calcium for its activity. Journal Of General Microbiology 1983, 129, 3565-3573.
  • J. H. Lakey,E.J.A. Lea. A method for analyzing the kinetics of membrane channels. Journal Of Physiology London 1983, 339, 84.

• Book Chapters

  • Clifton LA, Hall SCL, Mahmoudi N, Knowles TJ, Heinrich F, Lakey JH. Structural Investigations of Protein–Lipid Complexes Using Neutron Scattering. In: Kleinschmidt, JH, ed. Lipid-Protein Interactions: Methods and Protocols. New York: Humana Press Inc, 2019, pp.201-251.
  • Le Brun AP, Clifton LA, Holt SA, Holden PJ, Lakey JH. Deuterium Labeling Strategies for Creating Contrast in Structure-Function Studies of Model Bacterial Outer Membranes Using Neutron Reflectometry. In: Kumar CV, ed. Methods in Enzymology. San Diego: Academic Press Inc, 2017, pp.231-252.
  • Ridley H, Johnson CL, Lakey JH. Interfacial Interactions of Pore-Forming Colicins. In: Proteins: Membrane Binding and Pore Formation. Berlin: Springer, 2010, pp.81-90.
  • Anderluh G, Lakey JH. Lipid interactions of alpha-helical protein toxins. In: Tamm, L.K, ed. Protein-Lipid Interactions: From Membrane Domains to Cellular Networks. Weinheim, Germany: Wiley-VCH, 2005, pp.141-162.
  • Lakey JH, Gokce I. Protein-protein interactions. In: Encyclopedia of Life Sciences. London: John Wiley & Sons, 2001, pp.e-publication.
  • D. Jeanteur,J.H. Lakey,F. Pattus. The Porin Superfamily: Diversity and Common Features. In: J_M Ghuysen;R Hakenback, ed. Bacterial Cell Walls. Amsterdam: Elsevier, 1994, pp.363-379.
  • L Letellier,F.G. Van der Goot,J-M. Gonzalez-Manas,J.H. Lakey,W. Dowhan,F Pattus. Role of negatively charged phospholipids in the mode of action of negatively charged colicins: an attempt to relate in vitro to in vivo studies. In: A. Torriani-Gorini;E. Yagil;S. Silver, ed. Phosphate in Microorganisms: Cellular and Molecular biology. Washington DC: American Society for Microbiology, 1994, pp.239-244.
  • J. H. Lakey,M. W. Parker,J. M. Gonzalezmanas,D. Duche,G. Vriend,D. Baty,F. Pattus. Electrostatics and the Unfolding of colicins at the membrane surface. In: J Freer;R Aitken;J.E. Alouf;G Boulnois;P Falmagne;F Fehrenbach;C Montecucco;Y Piemont;R Rappuoli;T Wadstrom;B Witholt, ed. Bacterial Protein Toxins. Heidelberg: Gustav Fischer, 1994, pp.361-362.
  • J. Lakey,D. Baty,J.M. González-Mañas,D Duché,F Pattus. Site-directed fluorescence spectroscopy as a tool to study the membrane insertion of colicin A. In: Pattus James.R, F and Lazdunski,C, ed. Plasmid encoded toxins. Heidelberg: Springer Verlag, 1992, pp.127-138.
  • N. Didat,J-M. Gonzalez-Manas,F.G. Van der Goot,D. Jeanteur,J.H. Lakey,F. Pattus,D. Massotte,S. Scianimanico,H-U. Wilmsen. Puncturing Cell Membranes: Comparison of Colicin A and Aerolysin. In: A. Pullman, ed. Membrane proteins: Structures, interactions and models. Amsterdam: Kluwer Academic Publishers, 1992, pp.393-401.

• Conference Proceedings (inc. Abstracts)

  • Peters D, Ulusu Y, Waller H, Lakey J. Protein engineering of Caf1 from the plague bacterium Yersinia pestis for tissue engineering applications. In: 30th Anniversary Symposium of The Protein Society. 2016, Baltimore, MD: Wiley-Blackwell Publishing, Inc.
  • Waller H, Ulusu Y, Lakey JH. Caf1 of Yersinia pestis Forms Complex Highly Stable Protein Polymers and Hydrogel Scaffolds. In: Biophysical Society 60th Annual Meeting. 2016, Los Angeles, California: Cell Press.
  • Arunmanee W, Solovyova A, Johnson CL, Heenan RK, Lakey JH. Understanding Colicin N Import into Gram Negative Bacterial Cells using Small Angle Neutron Scattering. In: 58th Annual Meeting of the Biophysical-Society. 2014, San Francisco, CA: Cell Press.
  • Arunmanee W, Harris JR, Lakey JH. One and two dimensional arrays of membrane proteins stabilized by amphipol. In: Microscopy & Microanalysis 2014 Meeting. 2014, Hartford, CT: Cambridge University Press.
  • Johnson CL, Pengelly RJ, Ridley H, Lakey JH. Targeted Killing of Escherichia coli by an Unfolded Protein. In: 56th Annual Meeting of the Biophysical Society. 2012, San Diego, CA: Cell Press.
  • Huang Y, Le Brun A, Soliakov A, MacDonald C, Moore G, Lakey JH. Helix N-Cap Asp are the pH Trigger for Colicin a Membrane Insertion. In: 56th Annual Meeting of the Biophysical Society. 2012, San Diego, CA: Cell Press.
  • Collin M, Dickinson RE, Haniffa MA, McGovern N, Griffin H, Reynard L, Lakey J, Hambleton S, Santibanez-Koref MA, Bigley V. Evolution of Dendritic Cell, Monocyte B and NK Lymphoid (DCML) deficiency; a human primary immunodeficiency arising from GATA-2 mutation. In: European Congress of Immunology. 2012, Glasgow, UK: Wiley-Blackwell Publishing Ltd.
  • Hambleton S, McDonald DO, Morgan NV, Griffin H, Singh-Dang T, Grainger A, Reynard L, Gennery AR, Slatter M, Flood TJ, McKiernan P, Barge D, Abinun M, Hackett S, Loughlin J, Lakey J, Cant AJ, Santibanez-Koref M. Autosomal recessive combines immunodeficiency due to loss of function mutation in Tripeptidyl Peptidase II. In: 15th Biennial Meeting European Society for Immunodeficiency (ESID). 2012, Florence, Italy: Springer.
  • Johnson CL, Clifton LA, Solovyova A, Callow P, Weiss KL, Ridley H, Le Brun AP, Holt SA, Lakey JH. Small-Angle Neutron Scattering Reveals Colicin N Inserts into Clefts on the Outside of the OmpF Trimer. In: 55th Annual Meeting of the Biophysical Society. 2011, Baltimore, MD: Cell Press.
  • Dickinson RE, Bigley V, Griffin H, Lakey JH, McGovern N, Chua I, Wallis J, Hambleton S, Santibanez-Koref M, Collin M. Human dendritic cell deficiency is caused by GATA-2 mutation. In: Immunology: Annual Congress of the British Society for Immunology. 2011, Liverpool, UK: Wiley-Blackwell Publishing Ltd.
  • Chalton DA, Lakey JH. DE/SERVED Protein Analysis: Micro Scale Detection of Subtle Protein Stability Changes Using a Fluorescent Reporter. In: BIOPHYSICAL JOURNAL. 2010, 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA: CELL PRESS.
  • Bakrac B, Podlesek Z, Lakey JH, Werner A, Anderluh G. Equinatoxin, a eukaryotic pore-forming toxin used as a specific marker for cellular sphingomyelin. In: 34th FEBS Congress. 2009, Prague, Czech Republic: FEBS Journal: Wiley-Blackwell.
  • Kirmizidis G, Birch MA, Lakey JH. Osteoblast maturity dictates response to vascular endothelial growth factor. In: Calcified Tissue International: Abstracts of the 35th European Symposium on Calcified Tissues. 2008, Barcelona, Spain: Springer New York LLC.
  • Mitchell EA, Lakey JH, Birch M. Highly controlled surface presentation of protein signalling motifs to regulate bone formation. In: Bone Research Society Meeting. 2008, Manchester, UK: Calcified Tissue International: Springer.
  • Shah DS, Thomas MB, Phillips S, Cisneros DA, Le Brun AP, Holt SA, Lakey JH. Self-assembling layers created by membrane proteins on gold. In: Conference on Bionanotechnology - From Self-Assembly to Cell Biology. 2007, Homerton Coll, Cambridge: Biochemical Society Transactions, Portland Press Ltd.
  • Cooke MJ, Phillips S, Shah DS, Athey D, Lakey JH, Przyborski SA. Presentation of extracellular matrix motifs by biomimetic substrates to control cellular attachment and differentiation. In: Proceedings of the Anatomical Society of Great Britain and Ireland. 2007, University of Durham: Journal of Anatomy: Anatomical Society of Great Britain and Ireland.
  • Baboolal T, Lakey J. The colicin N/OmpF complex studied by electron microscopy and circular dichroism. In: Biophysical Journal: 48th Annual Meeting of the Biophysical Society. 2004, Baltimore, Maryland, USA: Cell Press.
  • Hong Q, Chalton DA, Visudtiphole V, Lakey JH. Omp F-ligand interactions measured in vivo by SPR. In: Biophysical Society 48th Annual Meeting. 2004, Baltimore, MD: Biophysical Journal: Biophysical Society.
  • Visudtiphole V, Thomas MB, Chalton D, Lakey JH. In vitro refolding of trimeric and dimeric OmpF. In: Biophysical Society 48th Annual Meeting. 2004, Baltimore, MD: Biophysical Journal: Biophysical Society.
  • Chalton DA, Lakey JH. Engineering a periplasmic turn of OmpF. In: Biophysical Society 47th Annual Meeting. 2003, San Antonio, Texas: Biophysical Journal: Biophysical Society.
  • Hong Q, Terrettaz S, Ulrich WP, Vogel H, Lakey JH. Assembly of pore proteins on gold electrodes. In: Biochemical Society Transactions. 2001, Bristol, UK: Portland Press Ltd.
  • Mobasheri H, Lakey JH, Lea EJA. Voltage-dependent gating in engineered OmpC porin channels using fluorescence probes. In: 44th Annual Meeting. 2000, New Orleans, USA: Cell Press.
  • Hong Q, Stora T, Dover LG, Vogel H, Lakey JH. Use of pore-forming proteins in Nanobiotechnology. In: 1st World Congress of High-Tech Medicine. 2000, Hanover, Germany: Medimond SRL.
  • Lakey JH, Gokce I, Hong Q. Protein-protein interactions during colicin N translocation. In: Abstracts of the 4th International Workshop on Pore-Forming Toxins. 2000, Trento, Italy: Springer.

• Note

  • Anderluh G, Macek P, Lakey JH. Peeking into a secret world of pore-forming toxins: Membrane binding processes studied by surface plasmon resonance. Toxicon 2003, 42(3), 225-228.

• Reviews

  • Lakey JH. Neutrons for biologists: a beginner's guide, or why you should consider using neutrons. Journal of the Royal Society Interface 2009, 6, S567-S573.
  • Anderluh G, Lakey JH. Disparate proteins use similar architectures to damage membranes. Trends in Biochemical Sciences 2008, 33(10), 482-490.
  • Besenicar M, Macek P, Lakey JH, Anderluh G. Surface plasmon resonance in protein-membrane interactions. Chemistry and Physics of Lipids 2006, 141(1-2), 169-178.
  • Popot J-L, Berry EA, Charvolin D, Creuzenet C, Ebel C, Engelman DM, Flotenmeyer M, Giusti F, Gohon Y, Herve P, Hong Q, Lakey JH, Leonard K, Shuman HA, Timmins P, Warschawski DE, Zito F, Zoonens M, Pucci B, Tribet C. Amphipols: Polymeric surfactants for membrane biology research. Cellular and Molecular Life Sciences 2003, 60(8), 1559-1574.
  • Fridd SL, Gokce I, Lakey JH. High level expression of His-tagged colicin pore-forming domains and reflections on the sites for pore formation in the inner membrane. Biochimie 2002, 84(5-6), 477-483.
  • Lakey JH, Slatin SL. Pore-forming colicins and their relatives. Current Topics in Microbiology and Immunology 2001, 257, 131-161.
  • Lakey JH, Slatin SL. Pore-forming colicins and their relatives. Current Topics in Microbiology and Immunology 2001, 257, 131-161.
  • Heyse S, Stora T, Schmid E, Lakey JH, Vogel H. Emerging techniques for investigating molecular interactions at lipid membranes. Biochimica et Biophysica Acta: Reviews on Biomembranes 1998, 1376(3), 319-338.