Aims

1.To introduce the concept of compartmentalisation within eukaryotic cells.
2.To explore in detail protein targeting to and transport across membranes, trafficking between organelles and to explore recurrent themes in these processes.
3.To provide examples of post-translational modifications that occur within the secretory pathway and how these affect trafficking and localisation of proteins.
4.To reveal the dynamic nature of membrane lipid and protein biochemistry and how it relates to cellular processes.
5.To examine the synthesis and topogenesis of membrane proteins.
6.To provide an understanding of key aspects of membrane structure and the physical constraints of the membrane environment.

Outline Of Syllabus

Biological membranes are critical for maintaining cellular function, but at the same time they impede the movement of molecules and proteins within the cell. The module will consider:

·the concepts of compartmentalisation within eukaryotic cells

·biological membranes including different types of membrane, e.g. viral, bacterial, archeal, sub-cellular and cellular

·micelles, bilayered liposomes and detergents

·recognition of membrane proteins and proteins that are transported into cellular organelles or secreted from the cell, from their polypeptide sequences

·how membrane and secreted proteins are synthesized, sorted and transported to the correct destinations

·the function of specific membrane and membrane-associated protein complexes.

Within the module emphasis will be placed on some of the common and recurrent themes by which proteins are delivered to their correct cellular location, the roles of molecular chaperones in protein trafficking and folding, sorting and recycling of proteins, quality control mechanisms that operate within the secretory pathway and post-translational modification of proteins.

Teaching Rationale And Relationship

The lectures will introduce and build upon previous exposure to the concepts in the learning outcomes. The workshop supports the timed summative assessment.

Some of the concepts and ideas covered in the lectures will be re-enforced in practical sessions which will give the students ‘hands on’ experience of purifying proteins.

One of the feedback sessions will be used to review the material covered in the practical (lab recording and reporting skills). The second will be used to discuss the formative assessment.

Assessment Rationale And Relationship

The written in person exams tests knowledge across the module.

The practical write-up assesses students understanding of key points in the lectures, ability to report and analyse results, and also their practical and team working skills (students will be working in groups of 2 or more for the practical).

The timed essay tests application of knowledge, understanding and critical evaluation skills.

The PIE-style SAQs will test the ability of students to interpret scientific data and prepare them for similar assessments in 3rd year and for their 3rd year dissertation.