Staff Profile
Dr Jacob Biboy
Research Associate
- Telephone: +44 (0) 191 208 3211
- Address: The Centre for Bacterial Cell Biology
Bioscience Institute
Medical School
Newcastle University
Richardson Road
Newcastle upon Tyne
NE2 4AX
United Kingdom
Publications
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Articles
- Liberini E, Fan S-H, Bayer AS, Beck C, Biboy J, Francois P, Gray J, Hipp K, Koch I, Peschel A, Sailer B, Vollmer D, Vollmer W, Gotz F. Staphylococcus aureus Stress Response to Bicarbonate Depletion. International Journal of Molecular Sciences 2024, 25(17), 9251.
- Sorlin AM, Lopez-Alvarez M, Biboy J, Gray J, Rabbitt SJ, Rahim JU, Lee SH, Bobba KN, Blecha J, Parker MF, Flavell RR, Engel J, Ohliger M, Vollmer W, Wilson DM. Peptidoglycan-Targeted [18F]3,3,3-Trifluoro-d-alanine Tracer for Imaging Bacterial Infection. JACS Au 2024, 4(3), 1039-1047.
- Dorrazehi GM, Winkle M, Desmet M, Stroobant V, Tanriver G, Degand H, Evrard D, Desguin B, Morsomme P, Biboy J, Gray J, Mitusinska K, Gora A, Vollmer W, Soumillion P. PBP-A, a cyanobacterial dd-peptidase with high specificity for amidated muropeptides, exhibits pH-dependent promiscuous activity harmful to Escherichia coli. Scientific Reports 2024, 14, 13999.
- Pohl S, Giacomelli G, Meyer FM, Kleeberg V, Cohen EJ, Biboy J, Rosum J, Glatter T, Vollmer W, van Teeseling MCF, Heider J, Bramkamp M, Thanbichler M. An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum. Nature Communications 2024, 15(1), 7616.
- Barnett MJ, Pinheiro J, Keown JR, Biboy J, Gray J, Lucinescu I-W, Vollmer W, Hirt RP, Simoes-Barbosa A, Goldstone DC. NlpC/P60 peptidoglycan hydrolases of Trichomonas vaginalis have complementary activities that empower the protozoan to control host-protective lactobacilli. PLoS Pathogens 2023, 19(8), e1011563.
- Frirdich E, Vermeulen J, Biboy J, Vollmer W, Gaynor EC. Multiple Campylobacter jejuni proteins affecting the peptidoglycan structure and the degree of helical cell curvature. Frontiers in Microbiology 2023, 14, 1162806.
- Izquierdo-Martinez A, Billini M, Miguel-Ruano V, Hernandez-Tamayo R, Richter P, Biboy J, Batuecas MT, Glatter T, Vollmer W, Graumann PL, Hermoso JA, Thanbichler M. DipM controls multiple autolysins and mediates a regulatory feedback loop promoting cell constriction in Caulobacter crescentus. Nature Communications 2023, 14(1), 4095.
- Lemos Rocha LF, Peters K, Biboy J, Depelteau JS, Briegel A, Vollmer W, Blokesch M. The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae. PLOS Genetics 2022, 18(3), e1010143.
- Garcia-Vello P, Tytgat HLP, Gray J, Elzinga J, D iLorenzo F, Biboy J, Vollmer D, De Castro C, Vollmer W, de Vos WM, Molinaro A. Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides. Glycobiology 2022, 32(8), 712-719.
- Kermani A, Biboy J, Vollmer D, Vollmer W. Outer-membrane anchoring enables LpoB to regulate peptidoglycan synthesis rate. The Cell Surface 2022, 8, 100086.
- Islam N, Kazi MI, Kang KN, Biboy J, Gray J, Ahmed F, Schargel R, Boutte CC, Dörr T, Vollmer W, Boll JM. Outer membrane integrity and peptidoglycan recycling promote carbapenem tolerance in Acinetobacter baumannii. mBio 2022, 13(3), e01001-22.
- Cobo NL, Sibinelli-Sousa S, Biboy J, Vollmer W, Bayor-Santos E, Prehna G. Molecular characterization of the type VI secretion system effector Tlde1a reveals a structurally altered LD-transpeptidase fold. Journal of Biological Chemistry 2022, 298(11), 102556.
- Banks EJ, ValdiviaDelgado M, Biboy J, Wilson A, Cadby IT, Vollmer W, Lambert C, Lovering AL, Sockett RE. Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria. Nature Communications 2022, 13, 1509.
- Radkov A, Sapiro AL, Flores S, Henderson C, Saunders H, Kim R, Massa S, Thompson S, Mateusiak C, Bibo J, Zhao Z, Starita LM, Hatleberg W, Vollmer W, Russell AB, Simorre JP, Anthony-Cahill S, Brzovic P, Hayes B, Chou S. Antibacterial potency of Type VI amidase effector toxins is dependent on substrate topology and cellular context. eLife 2022, 11, e79796.
- Mikkelsen K, Sirisarn W, Alharbi O, Alharbi M, Liu H, Nohr-Meldgaard K, Mayer K, Vestergaard M, Gallagher LA, Derrick JP, McBain AJ, Biboy J, Vollmer W, O'Gara JP, Grunert T, Ingmer H, Xia G. The Novel Membrane-Associated Auxiliary Factors AuxA and AuxB Modulate β-lactam Resistance in MRSA by stabilizing Lipoteichoic Acids. International Journal of Antimicrobial Agents 2021, 57(3), 106283.
- Kang K, Kazi M, Biboy J, Gray J, Bovermann H, Ausman J, Boutte C, Vollmer W, Boll J. Septal class A penicillin-binding protein activity and LD-transpeptidases mediate selection of colistin resistant lipooligosaccharide-deficient Acinetobacter baumannii. mBio 2021, 12(1), 1-16.
- Gurnani Serrano CK, Winkle M, Martorana AM, Biboy J, Morè N, Moynihan P, Banzhaf M, Vollmer W, Polissi A. ActS activates peptidoglycan amidases during outer membrane stress in Escherichia coli. Molecular Microbiology 2021, 116(1), 329-342.
- Sutton J, Carnell O, Lafage L, Gray J, Biboy J, Gibson J, Pollitt E, Tazoll S, Turnbull W, Hajdamowicz N, Salamaga B, Pidwill G, CCondliffe A, Renshaw S, Vollmer W, Foster SJ. Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction. PLOS Pathogens 2021, 17(3), e1009468.
- Hayes BM, Radkov AD, Yarza F, Flores S, Kim JR, Zhao Z, Lexa KW, Marnin L, Biboy J, Bowcutt V, Vollmer W, Pedra JHF, Chou S. Ticks resist skin commensals with immune factor of bacterial origin. Cell 2020, 183(6), 1562-1571.
- Liu X, Biboy J, Consoli E, Vollmer W, den Blaauwen T. MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA. PLoS Genetics 2020, 16(12), e1009276.
- Taylor JA, Bratton BP, Sichel SR, Blair KM, Jacobs HM, DeMeester KE, Kuru E, Gray J, Biboy J, VanNieuwenhze MS, Vollmer W, Grimes CL, Shaevitz JW, Salama NR. Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in Helicobacter pylori. eLife 2020, 9, e52482.
- Frirdich E, Biboy J, Pryjma M, Lee J, Huynh S, Parker CT, Girardin SE, Vollmer W, Gaynor EC. The Campylobacter jejuni helical to coccoid transition involves changes to peptidoglycan and the ability to elicit an immune response. Molecular Microbiology 2019, 112(1), 280-301.
- Morè N, Martorana AM, Biboy J, Otten C, Winkle M, Gurnani Serrano CK, Silva AM, Atkinson L, Yau H, Breukink E, den Blaauwen T, Vollmer W, Polissi A. Peptidoglycan remodeling enables E. coli to survive severe outer membrane assembly defect. mBio 2019, 10(1), e02729-18.
- Billini M, Biboy J, Kühn J, Vollmer W, Thanbichler M. A specialized MreB-dependent complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus. PLoS Genetics 2019, 15(2), e1007897.
- Jutras BL, Lochhead RB, Kloos ZA, Biboy J, Strle K, Gray J, Booth CJ, Govers SK, Vollmer W, Bockenstedt LK, Steere AC, Jacobs-Wagner C. Borrelia burgdorferi peptidoglycan is a persistent antigen in patients with Lyme arthritis. Proceedings of the National Academy of Sciences USA 2019, 116(27), 13498-13507.
- Pinheiro J, Biboy J, Vollmer W, Hirt RP, Keown JR, Artuyants A, Black MM, Goldstone DC, Simoes-Barbosa A. The Protozoan Trichomonas vaginalis Targets Bacteria with Laterally Acquired NlpC/P60 Peptidoglycan Hydrolases. mBio 2018, 9(6), e01784-18.
- Montón Silva A, Otten C, Biboy J, Breukink E, VanNieuwenhze M, Vollmer W, den Blaauwen T. The fluorescent D-amino acid NADA as a tool to study the conditional activity of transpeptidases in Escherichia coli. Frontiers in Microbiology 2018, (9), 2101.
- Carnell SC, Perry JD, Borthwick L, Vollmer D, Biboy J, Facchini M, Bragonzi A, Silipo A, Vergunst AC, Vollmer W, Khan ACM, De Soyza A. Targeting the bacterial cytoskeleton of the Burkholderia cepacia complex for antimicrobial development: A cautionary tale. International Journal of Molecular Sciences 2018, 19(6), 1604.
- Owen RA, Fyfe PK, Lodge A, Biboy J, Vollmer W, Hunter WN, Sargent F. Structure and activity of ChiX, a peptidoglycan hydrolase required for chitinase secretion by Serratia marcescens. Biochemical Journal 2018, 475(2), 415-428.
- Dembek M, Kelly A, Barwinska-Sendra A, Vollmer D, Biboy J, Gray J, Vollmer W, Salgado PS. Peptidoglycan degradation machinery in Clostridium difficile forespore engulfment. Molecular Microbiology 2018, 110(3), 390-410.
- Frirdich E, Biboy J, Huynh S, Parker CT, Vollmer W, Gaynor EC. Morphology heterogeneity within a Campylobacter jejuni helical population: the use of calcofluor white to generate rod-shaped C. jejuni 81-176 clones and the genetic determinants responsible for differences in morphology within 11168 strains. Molecular Microbiology 2017, 104(6), 948-971.
- Kuru E, Lambert C, Rittichier J, Till R, Ducret A, Derouaux A, Gray J, Biboy J, Vollmer W, VanNieuwenhze M, Brun YV, Sockett RE. Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation. Nature Microbiology 2017, 2, 1648-1657.
- Dewachter L, Verstraeten N, Jennes M, Verbeelen T, Biboy J, Monteyne D, Perez-Morga D, Verstrepen KJ, Vollmer W, Fauvart M, Michiels J. A mutant isoform of ObgE causes cell death by interfering with cell division. Frontiers in Microbiology 2017, 8, 1193.
- Peters K, Kannan S, Rao VA, Biboy J, Vollmer D, Erickson SW, Lewis RJ, Young KD, Vollmer W. The redundancy of peptidoglycan carboxypeptidases ensures robust cell shape maintenance in Escherichia coli. mBio 2016, 7(3), e00819-16.
- Jutras B, Scott M, Parry B, Biboy J, Gray J, Vollmer W, Jacobs-Wagner C. Lyme disease and relapsing fever Borrelia elongate through zones of peptidoglycan synthesis that mark division sites of daughter cells. Proceedings of the National Academy of Sciences of the USA 2016, 113(33), 9162-9170.
- Ha R, Frirdich E, Sychantha D, Biboy J, Taveirne ME, Johnson JG, DiRita VJ, Vollmer W, Clarke AJ, Gaynor EC. Accumulation of peptidoglycan O-acetylation leads to altered cell wall biochemistry and negatively impacts pathogenesis factors of Campylobacter jejuni. Journal of Biological Chemistry 2016, 291, 22686-22702.
- Chou S, Daugherty MD, Peterson SB, Biboy J, Yang YY, Jutras BL, Fritz-Laylin LK, Ferrin MA, Harding BN, Jacobs-Wagner C, Yang XF, Vollmer W, Malik HS, Mougous JD. Transferred interbacterial antagonism genes augment eukaryotic innate immune function. Nature 2015, 518(7537), 98-101.
- Gray AN, Egan AJF, van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar MAF, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W. Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. eLife 2015, 4, e07118.
- Egan AJF, Biboy J, van't Veer I, Breukink E, Vollmer W. Activities and regulation of peptidoglycan synthases. Philosophical Transactions of the Royal Society B 2015, 370(1679), 20150031.
- Frirdich E, Vermeulen J, Biboy J, Soares F, Taveirne ME, Johnson JG, DiRita VJ, Girardin SE, Vollmer W, Gaynor EC. Peptidoglycan LD-carboxypeptidase Pgp2 influences Campylobacter jejuni helical cell shape and pathogenic properties and provides the substrate for the DL-carboxypeptidase Pgp1. Journal of Biological Chemistry 2014, 289(12), 8007-8018.
- Egan AJF, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP. Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Proceedings of the National Academy of Sciences of the United States of America 2014, 111(22), 8197-8202.
- Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD. Identification, structure, and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair. Journal of Biological Chemistry 2013, 288, 26616-26624.
- Sycuro LK, Rule CS, Petersen TW, Wyckoff TJ, Sessler T, Nagarkar DB, Khalid F, Pincus Z, Biboy J, Vollmer W, Salama NR. Flow cytometry-based enrichment for cell shape mutants identifies multiple genes that influence Helicobacter pylori morphology. Molecular Microbiology 2013, 90(4), 869-883.
- Pilhofer M, Aistleitner K, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M, Jensen GJ. Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ. Nature Communications 2013, 4, 2856.
- Frirdich E, Biboy J, Adams C, Lee J, Ellermeier J, Gielda LD, DiRita VJ, Girardin SE, Vollmer W, Gaynor EC. Peptidoglycan-modifying enzyme pgp1 is required for helical cell shape and pathogenicity traits in Campylobacter jejuni. PLoS Pathogens 2012, 8(3), e1002602.
- Sycuro LK, Wyckoff TJ, Biboy J, Born P, Pincus Z, Vollmer W, Salama NR. Multiple peptidoglycan modification networks modulate Helicobacter pylori's cell shape, motility, and colonization potential. PLoS Pathogens 2012, 8(3), e1002603.
- Typas A, Banzhaf M, van den Berg van Saparoea B, Verheul J, Biboy J, Nichols RJ, Zietek M, Beilharz K, Kannenberg K, von Rechenberg M, Breukink E, den Blaauwen T, Gross CA, Vollmer W. Regulation of peptidoglycan synthesis by outer-membrane proteins. Cell 2010, 143(7), 1097-1109.
- Sycuro LK, Pincus Z, Gutierrez KD, Biboy J, Stern CA, Vollmer W, Salama NR. Peptidoglycan crosslinking relaxation promotes Helicobacter pylori's helical shape and stomach colonization. Cell 2010, 141(5), 822-833.
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Conference Proceedings (inc. Abstracts)
- Carnell SC, Perry JD, Vollmer D, Biboy J, Facchini M, Bragonzi A, Vergunst A, Vollmer W, Khan CMA, De Soyza A. Targeting the bacterial cytoskeleton of CF pathogens for antimicrobial development – A cautionary tale?. In: British Thoracic Society Winter Meeting. 2013, London, UK: BMJ Publishing Group.
- Carnell SC, Biboy J, Cerardi G, Ville B, Deleuse C, Samain R, Vollmer D, Khan CMA, Gray J, Vollmer W, De Soyza A. Pathogen associated molecular patterns in cystic fibrosis pathogens: Analysis of peptidoglycan structure. In: British Thoracic Society Winter Meeting. 2013, London, UK: BMJ Publishing Group.
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Review
- Taylor J, Santiago CC, Gray J, Wodzanowski KA, DeMeester KE, Biboy J, Vollmer W, Grimes CL, Salama NR. Localizing peptidoglycan synthesis in Helicobacter pylori using clickable metabolic probes. Current Protocols in Protein Science 2021, 1(4), e80.